Anticancer activity of Inula graveolensby induction of ROS-independent apoptosis and suppression of IL6-IL8 in cervical cancer cells

IF 0.7 4区医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas Pub Date : 2023-01-01 DOI:10.37360/blacpma.23.22.3.23

O. Yumrutas, I. Bozgeyik

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引用次数: 2

Abstract

Cancer is an important disease that causing to deaths in the world. Cervical cancer is one of the most common among women and must be treated quickly to prevent cell proliferation. Natural products have been used for cancer treatment due to their antiproliferative and apoptosis induction properties. In the present study, we aimed to determine the antiproliferative and apoptosis induction activities of methanol extract of Inula graveolens(IGME) in the human cerival cancer cell line (HeLa). Antiproliferative activity of IGME was evaluated by using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) method. Apoptosis induction activity was determined by AnnexinV/propodium iodide staining withFACS. ROS induction was determined by DCFH-DA (2′,7′-Dichlorofluorescin diacetate) staining. Interleukin 6 and 8 levels (IL-6 and IL-8) were determined by the Elisa method. IGME exhibited antiproliferative effect and induced apoptosis in the HeLa cells. IL-6, IL-8 and intracellular ROS levels were decreased after treatment of IGME. Consequently, IGME was found to have antiproliferative and apoptosis induction activities as an indicator of the anticancer activity.

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菊叶通过诱导ros非依赖性凋亡和抑制il - 6- il - 8的抗癌活性

癌症是世界上造成多人死亡的重要疾病。子宫颈癌是女性中最常见的疾病之一，必须迅速治疗以防止细胞增殖。由于其抗增殖和诱导细胞凋亡的特性，天然产物已被用于癌症治疗。在本研究中，我们旨在研究鸢尾草甲醇提取物(IGME)对人宫颈癌细胞系(HeLa)的抗增殖和诱导凋亡活性。采用MTT(3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四唑)法评价IGME的抗增殖活性。AnnexinV/propodium iodie染色法检测细胞凋亡诱导活性。采用DCFH-DA(2′，7′-二氯荧光素双醋酸酯)染色法检测ROS诱导情况。Elisa法检测白细胞介素6、8 (IL-6、IL-8)水平。IGME在HeLa细胞中表现出抗增殖和诱导凋亡的作用。IGME治疗后IL-6、IL-8及细胞内ROS水平降低。因此，我们发现IGME具有抗增殖和诱导细胞凋亡的活性，可以作为其抗癌活性的指标。

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来源期刊

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas PHARMACOLOGY & PHARMACY-

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas (BLACPMA), [Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants]; currently edited by the publishing house MS-Editions, is a bi-monthly international publication that publishes original peerreviewed research in the field of medicinal and aromatic plants, with nearly 20 years of experience. BLACPMA is a scientific journal that publishes two types of articles: Reviews (only in English) and Original Articles (Spanish or English), its main lines of action being agronomy, anthropology and ethnobotany, industrial applications, botany, quality and standardization, ecology and biodiversity, pharmacology, phytochemistry, pharmacognosy, regulatory and legislative aspects. While all areas of medicinal plants are welcome and the experimental approaches used can be broad and interdisciplinary; other areas of research that are not mentioned depend on the Editorial Committee for their acceptance.