Localization of Ebk/MDK1 in Mouse Oral Development

Abstract

The Ebk/MDK1 gene belongs to the receptor tyrosine kinase (RTKs) family and has been shown to be closely related to the Eph/Eck/Elk subfamily. A study was conducted to investigate the role of Ebk/ MDK1 in the oral development of early mouse embryogenesis. RT-PCR analysis identified the presence of Ebk/MDK1 mRNA in the region of the first molar at embryonic day 15 (E15). In situ hybridization analysis in the developing oral field from E12 to E16 revealed Ebk/MDK1 expressed in the mesenchyme underlying the dental epithelium of both of the first molars and incisors, as well as in the mesenchyme of the developing alveolar ridge, palate, tongue and lip. The distinct patterns of Ebk/MDK1 expression suggest that this gene may be involved in the establishment of these oral structures in mouse embryogenesis.