M. Atia, Ebtehal Sallam, H. Abdel-Tawab, A. Alghriany
{"title":"The Role of Fragmented Adipose Mesenchymal Stem Cells in Vitro Therapy for Human Hepatoma Cell Line Growth","authors":"M. Atia, Ebtehal Sallam, H. Abdel-Tawab, A. Alghriany","doi":"10.21608/aunj.2022.138653.1011","DOIUrl":null,"url":null,"abstract":"Background: Stem cells (SCs) from adipose tissue are being studied as part of regenerative medicine and used in vitro tissue engineering. Aim: The study goal was to highlight the modifications of fragmented adipose mesenchymal stem cells (AD-MSCs) on human hepatoma cell lines (HepG2 and Huh7) by inducing cell death (either apoptosis or necrosis). Materials and Methods: This study explains the methods of isolating, identifying, and characterization of fragmented frozen AD-MSCs. Also, investigate the phenotype of AD-MSCs culturing cells obtained in Passage 3 using CD105 & CD90 as positive markers and CD45 as a stem cells preferentially","PeriodicalId":8568,"journal":{"name":"Assiut University Journal of Multidisciplinary Scientific Research","volume":"26 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Assiut University Journal of Multidisciplinary Scientific Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/aunj.2022.138653.1011","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Stem cells (SCs) from adipose tissue are being studied as part of regenerative medicine and used in vitro tissue engineering. Aim: The study goal was to highlight the modifications of fragmented adipose mesenchymal stem cells (AD-MSCs) on human hepatoma cell lines (HepG2 and Huh7) by inducing cell death (either apoptosis or necrosis). Materials and Methods: This study explains the methods of isolating, identifying, and characterization of fragmented frozen AD-MSCs. Also, investigate the phenotype of AD-MSCs culturing cells obtained in Passage 3 using CD105 & CD90 as positive markers and CD45 as a stem cells preferentially