The Role of Fragmented Adipose Mesenchymal Stem Cells in Vitro Therapy for Human Hepatoma Cell Line Growth

M. Atia, Ebtehal Sallam, H. Abdel-Tawab, A. Alghriany
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Abstract

Background: Stem cells (SCs) from adipose tissue are being studied as part of regenerative medicine and used in vitro tissue engineering. Aim: The study goal was to highlight the modifications of fragmented adipose mesenchymal stem cells (AD-MSCs) on human hepatoma cell lines (HepG2 and Huh7) by inducing cell death (either apoptosis or necrosis). Materials and Methods: This study explains the methods of isolating, identifying, and characterization of fragmented frozen AD-MSCs. Also, investigate the phenotype of AD-MSCs culturing cells obtained in Passage 3 using CD105 & CD90 as positive markers and CD45 as a stem cells preferentially
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碎片化脂肪间充质干细胞在体外治疗人肝癌细胞系生长中的作用
背景:来自脂肪组织的干细胞作为再生医学的一部分正在被研究,并用于体外组织工程。目的:研究目的是强调碎片化脂肪间充质干细胞(AD-MSCs)通过诱导细胞死亡(凋亡或坏死)对人肝癌细胞系(HepG2和Huh7)的修饰作用。材料和方法:本研究解释了分离、鉴定和表征碎片化冷冻AD-MSCs的方法。此外,以CD105和CD90为阳性标记,优先以CD45为干细胞,研究第三代获得的AD-MSCs培养细胞的表型
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