Production of Chlorella sp. in a Designed Photobioreactor

Büsra Ak, E. Atak, M. Köse, O. Bayraktar
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引用次数: 1

Abstract

Click here for manuscript sample template Microalgae are photosynthetic microorganisms which are recently grown to produce biomass for food, pharmaceutical, dye, and bioenergy industries. Microalgae are the carbon source found in crude oil and natural gas. Over the years, there have been several advances in the design and operation of closed photobioreactors for microalgal cultures based on new reactor geometries as well as optimized aeration and mixing strategies. Closed photobioreactors ensure heat control, and high production through effective use of high-intensity light and prevent contamination in microalgae production. One of the most important factors that control cell growth in a photobioreactor is light availability. In this study, cultivation of Chlorella sp ., as microalgae were performed in a specially designed photobioreactor for productivity analysis and the pigment capacity analysis. The applied light energy was kept constant while applying either continuous or intermittent lighting during the growth of microalgae. The cultivation parameters were tested to find the optimal light mode as the continuous light or 12h light/ 12h dark cycle to maximize pigment amount. In order to determine the pigment amount in the cultivated algae extraction was done. Then by using UV spectrophotometer amount of chlorophyll a and b were determined in the obtained extracts.
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设计的光生物反应器中小球藻的生产
微藻是一种光合微生物,最近被用来为食品、制药、染料和生物能源工业生产生物质。微藻是原油和天然气中的碳源。多年来,基于新的反应器几何形状以及优化的曝气和混合策略,在微藻培养封闭光生物反应器的设计和操作方面取得了一些进展。闭式光生物反应器通过有效利用高强度光确保热控制和高产量,并防止微藻生产中的污染。控制光生物反应器中细胞生长的最重要因素之一是光的可用性。本研究在专门设计的光生物反应器中进行了小球藻微藻的培养,并进行了产率分析和色素容量分析。在微藻生长过程中,连续光照和间歇光照均保持恒定的光能。对培养参数进行了测试,确定了连续光照或12h光照/ 12h暗循环的最佳光照模式,以最大限度地提高色素含量。为了确定培养藻类中色素的含量,对其进行了提取。然后用紫外分光光度计测定提取液中叶绿素a和b的含量。
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