Analysis of correlation between nuclear DNA content, chromosome number, and flowering capacity of asymmetric somatic hybrids of diploid Solanum brevidens and (di) haploid S. tuberosum

The Japanese Journal of Genetics Pub Date : 1994-10-25 DOI:10.1266/JJG.69.525

J. Valkonen, K. Watanabe, E. Pehu

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引用次数: 22

Abstract

Selected stable standard Solanum genotypes and two populations of asymmetric somatic hybrids between Solanum brevidens (2n=2x=24) and dihaploid S. tuberosum (2n=2x=24) were analyzed to test the resolution of flow cytometric determination of nuclear DNA content (2C value) in terms of a loss or gain of chromosomes, and to analyze the correlation between the 2C value and the chromosome number. Correlation was high within the standard genotypes (r=0.99, P<0.001). However, the correlation was low within the two populations of somatic hybrids (r=0.54 and 0.65, 0.01

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二倍体短尾龙葵与单倍体龙葵不对称体细胞杂种核DNA含量、染色体数目与开花能力的相关性分析

分析了选择的稳定的标准龙骨基因型和龙骨(2n=2x=24)与二倍体龙骨(2n=2x=24)的两个非对称体细胞杂交群体，检验了流式细胞术测定核DNA含量(2C值)在染色体丢失或获得方面的分辨率，并分析了2C值与染色体数目的相关性。在标准基因型内相关性高(r=0.99, P<0.001)。而在两个体细胞杂交种群体内，相关性较低(r分别为0.54和0.65,0.01

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The Japanese Journal of Genetics

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