INVESTIGATION OF THE EFFECT OF DIFFERENT SOLVENT RATIOS IN THE CHEMOMETRIC PHYTO-EXTRACTION AND IN-VITRO SCREENING OF Carica papaya FRUIT, USING RESPONSE SURFACE METHODOLOGY [RSM]

Abstract

Carica papaya plant is a popular medicinal plant with notable anti-parasitic potentials. This work reports chemometrics of the three solvents [petroleum ether, chloroform and methanol] in the in-vitro screening of Plasmodium falciparum and Trypanosome brucei b. The Response Surface Methodology [RSM] method based on a second order and three variables Box-Behnken Design (BBD) was employed to obtain the optimal combination of extraction conditions. The extraction process parameters of the fruit of Carica papaya were optimized by BBD so as to get maximal yield of bio-actives. Different solvent ratios were used to achieve different response optimized by chemometric. For the in-vitro screening of Plasmodium falciparum and Trypanosome brucei b., the extracts of Carica papaya fruit were subjected to assay procedures to see their viability. Summarily, the 3D response surface plot and the contour plot derived from the mathematical models were applied to determine the optimal conditions. Under the optimized conditions, the experimental value of 77.97% viability on Plasmodium f. was in close agreement with the value predicted (77.97 ± 0.01 %) by the model and 82.86 ± 1.10%was predicted for Trypanosome brucei b. with an experimented value of 83.48%; showing close correlation between the predicted and experimental values. Considering RSM studies, it was found that the mixture of petroleum ether, chloroform and methanol solvents at a ratio of 55 mL: 27.50 mL: 17.50 mL gave an optimum viability on the in-vitro screening. Hence, the polarity of solvent affects the yield of bioactive compounds in the plant material.