Effect of ACE Plus Selenium on Total Antioxidant/Oxidant Capacity and Nitric Oxide Levels in Rabbits

Abstract

: Pills including vitamins and minerals have been used as part of a sedentary lifestyle, malnutrition, aging, and age-related illnesses in developed countries. This study specifically focuses on the effect of ACE Plus Selenium given rabbits through intraperitoneal (i.p) injection on their total oxidant, antioxidant capacity (TOC, TAC), and nitric oxide (NO) levels. In this study, 0,5 ml/kg of normal saline was injected into rabbits in the control group and 0,5 ml/kg of ACE Plus Selenium was conducted to the treatment group twice (every other day) via i.p. route. Following the injection, plasmas of blood samples obtained in the second and fourth days were separated, and stored at –20 º C until the analysis. Plasma TAC, TOC and NO levels were determined spectrophotometrically. The TOC, TAC and NO levels of the rabbits were compared to the control group statistically. While TOC levels were observed to decrease (p<0,05) on the fourth day, the NO levels increased (p<0,01) on the second day and became normal on the fourth day. Further no statistical alteration was observed in the TAC levels. As a result, it can be concluded that ACE Plus Selenium had no effect on TAC level, it may decrease TOC levels in parallel to the decline in oxidative stress; further, it can increase NO levels acutely as a result of α-tocopherol and ascorbate ingredients.