Natural substrates for extracellular lipase production from Pseudomonas aeruginosa HE05 isolated from diesel oil contaminated soil

Abstract

The present work focused on the production of extracellular lipase from Pseudomonas aeruginosa HE05 emplying natural oils of plants as substrat. Nine isolates (HE01-HE09) were isolated from the desiel oil contaminated soil and screened for lipase activity on nutrient agar medium supplemented with 1% olive oil and tween 80. Pseudomonas aeruginosa HE05 was identified as the hyperproducer of lipase. Maximum lipase production (3123U/ml) was recorded when 1% olive oil and 1% tween 80 added in nutrient broth used as a substrate in submerged fermentation. The optimal production was achevied in 96 hrs at 37C at 100 rpm. Quantification of lipase units were performed by turbidimetric assay. Supplementation of nutrient broth with natural oils was found as inducer as well as substrate for the production of lipase. Among the natural oils, mustard oil, corn oil, almond oil are better substrate for extracellular lipase production.