Micropropagation of belarusian cultivars of blue honeysuckle (Lonicera caerulea L. var. kamtschatica)

Abstract

For blue honeysuckle cultivars of Belarusian selection (Zinri and Sinyavokaya), a method of micropropagation for the production of high-quality planting material is proposed. Initiation of in vitro culture: in the period of intensive growth of shoots – the first decade of June. Explants were apical points isolated from the apical and axillary buds of green shoots. The nutrient medium was WPM supplemented with 6-BA at a concentration of 1.0 mg/l. Micropropagation stage: MS medium containing double strength iron chelate with 1.5 mg/l of 6-BA. In vitro rhizogenesis: in MS medium the concentration of macroand microsalts and iron chelate was reduced to half strength, the sucrose concentration was reduced to 20 g/l, IBA – 1.5–2.0 mg/l). Ex vitro adaptation: substrate – perlite. It is possible to reduce the cost of obtaining the planting material by eliminating in vitro rooting stage from micropropagation scheme. Simultaneous direct ex vitro rooting and adaptation of microplants should be carried out on the substrate of Sphagnum L. moss with a surface layer (0.5 cm) of peat. Long-term storage (up to 12 months) at low positive temperatures (+3–4 °C) in refrigerator should be carried out at rooting stage (in MS medium the concentration of macro- and microsalts and iron chelate was reduced to half strength, the sucrose concentration was reduced to 20 g/l, IBA – 1.0 mg/l).