{"title":"Physicochemical Properties of four Phosphate-solubilizing Bacteria Isolated from the Semi-arid Regions of North Gujarat, India","authors":"Anurag Yadav, Ganvani Hinaben, K. Yadav, R. Ahmad","doi":"10.21921/jas.v9i03.11008","DOIUrl":null,"url":null,"abstract":"The rhizosphere of several plants was screened for phosphate solubilizing bacteria (PSB) from eight locations of North Gujrat. Thirty-three PSB were isolated in the study. Four PSB isolates with the highest phosphate solubilization index between 1.55–2 were characterized morphologically and biochemically and identified through 16S rRNAsequencing as Klebsiella aerogenes, Klebsiella pneumoniae, Kocuria flava and Enterobacter hormaechei. The growth of isolates was measured by plotting an optical density-based semi-logarithmic growth curve. The isolates were measured for Psolubilization in Pikovskaya broth. The isolate K. flava LC515414 -1solubilized maximum CaPO (7.63 μm P mL). The acid and alkaline phosphatase activity of 34the isolates was also measured for 96 h in the growth medium. After 96 h of growth, the isolate -1K. flava LC515414 had the highest final acid and alkaline phosphatase activity of 4.68 U Land -15.67 U L","PeriodicalId":14972,"journal":{"name":"Journal of AgriSearch","volume":"7 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of AgriSearch","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21921/jas.v9i03.11008","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The rhizosphere of several plants was screened for phosphate solubilizing bacteria (PSB) from eight locations of North Gujrat. Thirty-three PSB were isolated in the study. Four PSB isolates with the highest phosphate solubilization index between 1.55–2 were characterized morphologically and biochemically and identified through 16S rRNAsequencing as Klebsiella aerogenes, Klebsiella pneumoniae, Kocuria flava and Enterobacter hormaechei. The growth of isolates was measured by plotting an optical density-based semi-logarithmic growth curve. The isolates were measured for Psolubilization in Pikovskaya broth. The isolate K. flava LC515414 -1solubilized maximum CaPO (7.63 μm P mL). The acid and alkaline phosphatase activity of 34the isolates was also measured for 96 h in the growth medium. After 96 h of growth, the isolate -1K. flava LC515414 had the highest final acid and alkaline phosphatase activity of 4.68 U Land -15.67 U L
从古吉拉特邦北部8个地点对几种植物的根际进行了磷酸盐增溶菌(PSB)筛选。本研究共分离到33株PSB。磷酸溶解指数在1.55 ~ 2之间最高的4株PSB菌株进行了形态和生化鉴定，并通过16S rrna测序鉴定为产气克雷伯菌、肺炎克雷伯菌、黄化kcuria和霍氏肠杆菌。通过绘制基于光密度的半对数生长曲线来测量分离株的生长。测定了分离菌在Pikovskaya肉汤中的增溶性。菌株LC515414 -1最大溶出CaPO (7.63 μm P mL)。测定了34株菌株在生长培养基中96 h的酸性和碱性磷酸酶活性。生长96 h后，分离物-1K。黄酮LC515414的最终酸碱性磷酸酶活性最高，为4.68 U Land -15.67 U L
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