Protective Effects of Alpha-lipoic Acid and Melatonin Against Cadmium-induced Oxidative Stress in Erythrocytes of Rats

Samy Ali Hussei, Omnia, M. Abd El-Ham, Ahmad M. Sabry F
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引用次数: 15

Abstract

Cadmium (Cd) is a well-known human carcinogen and a potent nephrotoxin. The protective effects of alpha-lipoic acid and melatonin against cadmium (Cd) induced oxidative stress to erythrocytes in rats were evaluated. One hundred male albino rats were divided into five groups containing 20 rats each. Group I: (control) administered distilled water. Group II :( Cadmium exposed group) received cadmium chloride (4.4 mg/kg body weight of 1/20th of LD50 / day) orally for 10 weeks. Group III :( Cadmium +alpha-lipoic acid) received cadmium chloride (4.4 mg/kg body weight) and treated daily with alphalipoic acid (54 mg/kg body weight/ i.p). Group IV :( Cadmium +Melatonin) received cadmium chloride (4.4 mg/kg body weight) and treated daily with melatonin (10 mg/kg body weight/orally). Group V :( Cadmium +alpha-lipoic acid and melatonin). Urea and creatinine concentrations were determined in plasma. However, erythrocyte hemolysate were processed for the determination of L-Malondialdhyde (LMDA), catalase (CAT), superoxide dismutase (SOD), GlutathioneS-transferase (GST) and reduced Glutathione (GSH) in addition to erythrocytes Glucose -6-phosphate dehydrogenase (G-6-PDH) activity. Also, kidney specimens were used for cadmium residues determination. The obtained results revealed that, a significant increase in plasma urea, creatinine concentrations and erythrocyte L-MDA level, SOD activity in addition to kidney cadmium residue concentrations were observed in cadmium intoxicated rats. However, administration of alpha-lipoic acid, melatonin and their combination exhibited a significant decreased in all mentioned parameters. On the other hand, a significant decreased in erythrocyte CAT, GST and G-6-PDH activities, GSH concentration were observed in cadmium intoxicated rats. Meanwhile, treatment with alpha-lipoic acid and melatonin resulted in significant increase in all mentioned parameters. It could be concluded that, the potential protective effect of alpha-lipoic acid and melatonin as a powerful agents and may be useful as an antioxidants in combating free radical-induced oxidative stress and tissue injury that is a result of cadmium toxicity.
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α -硫辛酸和褪黑素对镉诱导大鼠红细胞氧化应激的保护作用
镉(Cd)是一种众所周知的人类致癌物和强效肾毒素。研究了α -硫辛酸和褪黑素对镉(Cd)诱导的大鼠红细胞氧化应激的保护作用。将100只雄性白化大鼠分为5组,每组20只。第一组:(对照)给予蒸馏水。II组(镉暴露组)口服氯化镉(4.4 mg/kg体重,LD50的1/20 /天),持续10周。第三组(镉+ α -硫辛酸):给予氯化镉(4.4 mg/kg体重),每日给予α -硫辛酸(54 mg/kg体重/ i.p)。IV组(镉+褪黑素):给予氯化镉(4.4 mg/kg体重),每日给予褪黑素(10 mg/kg体重/口服)。V组:(镉+ α -硫辛酸和褪黑素)。测定血浆尿素和肌酐浓度。然而,处理红细胞溶血液以测定l -丙二醛(LMDA)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽转移酶(GST)和还原性谷胱甘肽(GSH)以及红细胞葡萄糖-6-磷酸脱氢酶(G-6-PDH)活性。此外,肾脏标本用于镉残留测定。结果表明,镉中毒大鼠血浆尿素、肌酐浓度、红细胞L-MDA水平、SOD活性及肾脏镉残留浓度均显著升高。然而,α -硫辛酸、褪黑激素及其联合用药在所有上述参数中均显著降低。另一方面,镉中毒大鼠红细胞CAT、GST、G-6-PDH活性和GSH浓度显著降低。与此同时,α -硫辛酸和褪黑素治疗导致上述参数显著增加。由此可见,α -硫辛酸和褪黑素作为一种强有力的抗氧化剂,在对抗镉中毒引起的自由基诱导的氧化应激和组织损伤方面具有潜在的保护作用。
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