The effectiveness of the Culture for Dientamoeba fragilis from the Stool

Ö. Kurt, N. Girginkardesler, A. Özbilgin, Ü. Z. Ok
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Abstract

Objective: In the identification of Dientamobea fragilis, Polymerase chain reaction (PCR) is by far more sensitive than microscopic examination of permanent-stained smears, while it is faster and less labour-intensive than culture; however, it is expensive. We aimed to assess the sensitivity of culture and compare the efficacies of three culture media: Robinson’s medium (RM), Dobell’s medium (DM) and Talis’s Medium (TM), for the identification of D. fragilis. Materials and Methods: Stool samples of 104 individuals admitted to Manisa Celal Bayar University Hospital with complaints such as abdominal pain, itching, meteorism and diarrhea, and no detection of D. fragilis in the initial examination of trichrome-stained smears of their stools, were cultivated in RM, DM and TM on the same day. Results: Trophozoites of D. fragilis were detected in 11 of 104 (10.6%) samples, where all were positive in RM, while 3 and 4 of 11 were also positive in DM and TM, respectively. Two samples were found to be positive in all three media. RM was by far the leading medium to supply vast numbers of trophozoites. Conclusion: These results indicate that culture is more effective than microscopic examination of permanent stained smears of stools to identify D. fragilis when PCR is not available, and RM could be the first choice for culture as it supplies huge amounts of trophozoites useful for further molecular studies.
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粪便中脆弱地阿米巴培养的效果
目的:聚合酶链反应(PCR)在鉴定脆弱双塔莫菌时,灵敏度远高于永久染色涂片显微镜检查,而比培养更快,劳动强度更低;然而,它是昂贵的。我们的目的是评估培养的敏感性,并比较三种培养基:罗宾逊培养基(RM)、多贝尔培养基(DM)和塔利斯培养基(TM)对脆性裂裂菌的鉴定效果。材料与方法:将104例以腹痛、瘙痒、头晕、腹泻等症状就诊于马尼萨·塞拉尔·巴亚尔大学医院,粪便三色涂片初检未检出脆弱梭菌的患者,于同一天在RM、DM和TM进行培养。结果:104份样品中检出脆弱单胞菌滋养体11份(10.6%),均为RM阳性,DM阳性3份,TM阳性4份。两份样本在所有三种培养基中均呈阳性。到目前为止,RM是提供大量滋养体的主要介质。结论:这些结果表明,在没有PCR的情况下,培养比显微镜检查粪便永久染色涂片更有效地鉴定脆弱单胞菌,RM可作为培养的首选,因为它提供了大量的滋养体,可用于进一步的分子研究。
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