Y. Shin, Jungwoo Kim, Dazy Johnson, A. Dooraghi, Wilson X. Mai, Lisa Ta, A. Chatziioannou, M. Phelps, D. Nathanson, J. Heath
{"title":"Quantitative assessments of glycolysis from single cells.","authors":"Y. Shin, Jungwoo Kim, Dazy Johnson, A. Dooraghi, Wilson X. Mai, Lisa Ta, A. Chatziioannou, M. Phelps, D. Nathanson, J. Heath","doi":"10.1142/S2339547815200058","DOIUrl":null,"url":null,"abstract":"The most common positron emission tomography (PET) radio-labeled probe for molecular diagnostics in patient care and research is the glucose analog, 2-deoxy-2-[F-18]fluoro-D-glucose (18F-FDG). We report on an integrated microfluidics-chip/beta particle imaging system for in vitro18F-FDG radioassays of glycolysis with single cell resolution. We investigated the kinetic responses of single glioblastoma cancer cells to targeted inhibitors of receptor tyrosine kinase signaling. Further, we find a weak positive correlation between cell size and rate of glycolysis.","PeriodicalId":82888,"journal":{"name":"Technology (Elmsford, N.Y.)","volume":"93 1","pages":"172-178"},"PeriodicalIF":0.0000,"publicationDate":"2015-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Technology (Elmsford, N.Y.)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1142/S2339547815200058","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
The most common positron emission tomography (PET) radio-labeled probe for molecular diagnostics in patient care and research is the glucose analog, 2-deoxy-2-[F-18]fluoro-D-glucose (18F-FDG). We report on an integrated microfluidics-chip/beta particle imaging system for in vitro18F-FDG radioassays of glycolysis with single cell resolution. We investigated the kinetic responses of single glioblastoma cancer cells to targeted inhibitors of receptor tyrosine kinase signaling. Further, we find a weak positive correlation between cell size and rate of glycolysis.
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