Effectiveness of Common Disinfecting Agents against Isolates of Nannizziopsis guarroi

B. Jourdan, Caitlin Hemby, M. Allender, Ivana Levy, Eliana Foltin, K. Keller
{"title":"Effectiveness of Common Disinfecting Agents against Isolates of Nannizziopsis guarroi","authors":"B. Jourdan, Caitlin Hemby, M. Allender, Ivana Levy, Eliana Foltin, K. Keller","doi":"10.5818/JHMS-D-22-00004","DOIUrl":null,"url":null,"abstract":"Abstract Nannizziopsis guarroi, a keratinophilic fungus, is an important cause of dermatomycosis in companion lizards. At present, effective disinfection protocols are unknown and additional information is needed to prevent contamination of surfaces and equipment used in the care of these animals. To this aim, the qualitative in vitro–disinfecting capability of eight commonly used household and laboratory disinfectants (Novalsan®, 3% and 10% dilutions of commercial bleach, Virkon®-S, Lysol® household cleaner with hydrogen peroxide, 70% ethanol, 409®, and household cleaning ammonia) were tested at two different contact times (2 and 10 min) with three different aqueous fungal concentrations of four molecularly confirmed N. guarroi isolates. A positive control after contact with saline was also grown. After contact with disinfectant or saline, the isolates were incubated, and photographic images were taken of plate growth on day 10. Images of each plate were scored using a semiquantitative scoring system. The only disinfectant that completely inhibited growth for all four isolates at both contact times and at all three isolate dilutions was the 10% dilution of commercial bleach. All four isolates grew after contact with ammonia, regardless of contact time or isolate dilution, and the other disinfectants showed variable inhibition of growth that was either isolate or concentration dependent, or both. In conclusion, a minimum of 2 min of exposure to a 10% dilution of commercial bleach is recommended for disinfection of surfaces and instruments contaminated with N. guarroi.","PeriodicalId":16054,"journal":{"name":"Journal of Herpetological Medicine and Surgery","volume":"31 1","pages":"40 - 44"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Herpetological Medicine and Surgery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5818/JHMS-D-22-00004","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4

Abstract

Abstract Nannizziopsis guarroi, a keratinophilic fungus, is an important cause of dermatomycosis in companion lizards. At present, effective disinfection protocols are unknown and additional information is needed to prevent contamination of surfaces and equipment used in the care of these animals. To this aim, the qualitative in vitro–disinfecting capability of eight commonly used household and laboratory disinfectants (Novalsan®, 3% and 10% dilutions of commercial bleach, Virkon®-S, Lysol® household cleaner with hydrogen peroxide, 70% ethanol, 409®, and household cleaning ammonia) were tested at two different contact times (2 and 10 min) with three different aqueous fungal concentrations of four molecularly confirmed N. guarroi isolates. A positive control after contact with saline was also grown. After contact with disinfectant or saline, the isolates were incubated, and photographic images were taken of plate growth on day 10. Images of each plate were scored using a semiquantitative scoring system. The only disinfectant that completely inhibited growth for all four isolates at both contact times and at all three isolate dilutions was the 10% dilution of commercial bleach. All four isolates grew after contact with ammonia, regardless of contact time or isolate dilution, and the other disinfectants showed variable inhibition of growth that was either isolate or concentration dependent, or both. In conclusion, a minimum of 2 min of exposure to a 10% dilution of commercial bleach is recommended for disinfection of surfaces and instruments contaminated with N. guarroi.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
常用消毒剂对瓜状芽孢杆菌分离株的效果研究
摘要:瓜南芽孢菌(Nannizziopsis guarroi)是一种嗜角真菌,是引起同伴蜥蜴皮肤真菌病的重要原因。目前,有效的消毒方案尚不清楚,需要更多的信息来防止用于护理这些动物的表面和设备受到污染。为此,在两种不同的接触时间(2分钟和10分钟)下,对八种常用的家用和实验室消毒剂(Novalsan®、3%和10%稀释的商用漂白剂、Virkon®-S、含双氧水、70%乙醇、409®和家用清洁氨的Lysol®家用清洁剂)与四种分子确认的N. guarroi分离物的三种不同水溶真菌浓度进行定性体外消毒能力测试。与生理盐水接触后也培养了阳性对照。分离菌与消毒剂或生理盐水接触后孵育,第10天拍摄平板生长照片。使用半定量评分系统对每个板块的图像进行评分。在所有接触次数和所有三种分离物稀释度下,唯一完全抑制所有四种分离物生长的消毒剂是10%稀释的商业漂白剂。与接触时间或分离物稀释度无关,所有四种分离物在接触氨后都能生长,而其他消毒剂对生长的抑制作用不同,要么是分离物抑制,要么是浓度抑制,或者两者兼而有之。总之,建议在10%稀释的商用漂白剂中至少暴露2分钟,以消毒被金翅虫污染的表面和仪器。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Preliminary Results of Variation in Plasma N-acetyl-beta-D-glucosaminidase Concentration Among Bearded Dragons (Pogona vitticeps), Corn Snakes (Pantherophis guttatus), and Wood Turtles (Glyptemys insculpta) Prevalence of Urolithiasis in Client-owned Chelonians and its Association with Selected Housing and Dietary Parameters in African Spurred Tortoises (Centrochelys sulcata) Presumptive Stenotrophomonas maltophilia Associated Multisystemic Granulomatous Disease in Aquatic Turtles Successful Surgical Repair of a Spontaneous Arterial Aneurysm in an Inland Bearded Dragon (Pogona vitticeps) Two-dimensional and Doppler Echocardiographic Findings in Healthy Non-sedated Captive European Pond Turtles (Emys orbicularis)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1