Expression of matrix metalloproteinases (MMPs) and their inhibitor (TIMP) genes on mRNA and protein levels in oral squamous cell carcinoma

Q4 Medicine Nowotwory Pub Date : 2021-02-05 DOI:10.5603/NJO.2021.0003
Artur Wróbel-Roztropiński, B. Zielińska-Kaźmierska, Hubert Roztropiński, Weronika Lucas-Grzelczyk, J. Szemraj, M. Józefowicz-Korczynska
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引用次数: 1

Abstract

Introduction. To investigate the mRNA and protein expression of MMP-2, MMP-9, MMP-7 and their tissue inhibitor TIMP-2 in tissue specimens with oral squamous cell cancer (OSCC) and in healthy tissues. Material and methods. The expression genes of MMP-2, MMP-9, MMP-7 and TIMP-2 on mRNA levels were detected by the RT-PCR method in 31 samples with oral squamous cell carcinoma and in 31 healthy, control tissues. Secondly, the concentration of the analysed metalloproteinases and their inhibitor was assessed in tumor and non-tumor tissues using the enzyme-linked immunosorbent assay (ELISA) method. Results. The mean values of gene expression of MMP-2, MMP-7, MMP-9 and TIMP in tissues with oral squamous cell cancer were significantly higher in comparison to normal ones (p < 0.0001). Similar observations were found for concentration levels of analysed MMPs and TIMP in tissues with and without oral cancer (p < 0.0001). Conclusions. The present study demonstrated that MMP-2, MMP-7, MMP-9 and TIMP-2 gene expression on protein and mRNA levels is higher in oral squamous cell carcinoma tissues than in healthy control tissues. This may suggest that MMPs and TIMP play an important role in tumorogenesis. We did not observe any correlation between the clinicopathological characteristics of patients with OSCC and expression levels of MMPs and TIMP.
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基质金属蛋白酶(MMPs)及其抑制剂(TIMP)基因在口腔鳞状细胞癌组织中mRNA和蛋白水平的表达
介绍。探讨口腔鳞状细胞癌(OSCC)组织标本和健康组织中MMP-2、MMP-9、MMP-7及其组织抑制剂TIMP-2的mRNA和蛋白表达。材料和方法。采用RT-PCR方法检测31例口腔鳞状细胞癌和31例健康对照组织中MMP-2、MMP-9、MMP-7和TIMP-2 mRNA水平的表达。其次,采用酶联免疫吸附试验(ELISA)方法测定肿瘤和非肿瘤组织中金属蛋白酶及其抑制剂的浓度。结果。口腔鳞状细胞癌组织中MMP-2、MMP-7、MMP-9和TIMP基因表达的平均值明显高于正常组织(p < 0.0001)。在有和没有口腔癌的组织中,所分析的MMPs和TIMP的浓度水平也发现了类似的观察结果(p < 0.0001)。结论。本研究表明,口腔鳞状细胞癌组织中MMP-2、MMP-7、MMP-9和TIMP-2基因在蛋白和mRNA水平上的表达均高于健康对照组织。这可能表明MMPs和TIMP在肿瘤发生中起重要作用。我们没有观察到OSCC患者的临床病理特征与MMPs和TIMP的表达水平之间的任何相关性。
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来源期刊
Nowotwory
Nowotwory Medicine-Oncology
CiteScore
0.90
自引率
0.00%
发文量
44
期刊介绍: NOWOTWORY Journal of Oncology publishes papers which cover all aspects of oncology but concentrates on clinical studies, both research orientated and treatment orientated, rather than on laboratory studies. Contributions are also welcomed from the fields of epidemiology, tumor pathology, radiobiology and radiation physics.
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