Comparison of C-banding patterns and in situ hybridization sites using highly repetitive and total genomic rye DNA probes of `Imperial' rye chromosomes added to `Chinese Spring' wheat

Abstract

C-banding patterns of the seven `Imperial' rye chromosomes and 11 derived rye telocentrics added to `Chinese Spring' were analyzed and compared with in situ hybridization (ISH) patterns using biotin labeled highly repetitive rye DNA sequences pSc119, pSc74, and total rye genomic DNA as probes. C-banding and ISH analyses allow the identification of all individual rye chromosomes and most chromosome arms with these probes. A C-banded karyotype of the added rye chromosomes was constructed and data on chromosome lengths, arm ratios, and fraction lengths of diagnostic C-bands are given following the nomenclature system recently recommended for wheat. Similar telomeric and interstitial localized ISH patterns were observed with pSc119, pSc74, and total rye genomic DNA probes. In addition, pSc119 and total rye genomic DNA labels rye chromatin over their entire lengths, also permitting the detection of unbanded euchromatic rye chromatin transfers in wheat. All localized ISH sites correspond to dark C-banded regions; however, not all C-bands show up as hybridization sites.