新規セルロースアセテート膜'セレカ-VSP'の血清タンパク分画検査への評価 : ロット間差および施設間差の多施設共同研究

弘子 渡邊, 綾 杉浦, 和子 大竹, 皓子 大竹, 深田 比呂子, 康司 堀井, 満 村田, 和代 中村, 秋恵 須郷, 栄治 宮島, 京子 宮﨑, 正芳 米山, 照夫 江上, 卓 渡邊, 芝 紀代子, 真人 前川
{"title":"新規セルロースアセテート膜'セレカ-VSP'の血清タンパク分画検査への評価 : ロット間差および施設間差の多施設共同研究","authors":"弘子 渡邊, 綾 杉浦, 和子 大竹, 皓子 大竹, 深田 比呂子, 康司 堀井, 満 村田, 和代 中村, 秋恵 須郷, 栄治 宮島, 京子 宮﨑, 正芳 米山, 照夫 江上, 卓 渡邊, 芝 紀代子, 真人 前川","doi":"10.2198/SBK.51.135","DOIUrl":null,"url":null,"abstract":"In order to substitute SELECA-VSP for SEPARAX-SP, we investigated membrane performance difference among SELECA-VSP lots and among laboratories. We examined three lots of SELECA-VSP, namely T10, T12 and T14. Automated electrophoresis system for protein fraction test used are Olympus AES series in two laboratories and JOKOH CTE series in other two laboratories. The 4 laboratories assayed common serum samples as usual procedure of each laboratory.Among different membrane lots, good correlation between each two lot was observed in the five serum protein fractions. There was statistically significant difference among the membrane lots, however, it might be only due to good reproducibility and statistics. The bias was below clinical permission limit.Among laboratories, good correlation between each two laboratory was observed in the five serum protein fractions. The bias between laboratories is below permission limit, compared with bias detected from external quality assessment program for SEPARAX-SP.In conclusion, because the bias in membrane lots and laboratories can be ignored for laboratory diagnosis of serum protein fraction test, SELECA-VSP will come onto the market to make up for SEPARAX-SP.","PeriodicalId":15059,"journal":{"name":"Journal of capillary electrophoresis","volume":"28 1","pages":"135-145"},"PeriodicalIF":0.0000,"publicationDate":"2007-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of capillary electrophoresis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2198/SBK.51.135","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

In order to substitute SELECA-VSP for SEPARAX-SP, we investigated membrane performance difference among SELECA-VSP lots and among laboratories. We examined three lots of SELECA-VSP, namely T10, T12 and T14. Automated electrophoresis system for protein fraction test used are Olympus AES series in two laboratories and JOKOH CTE series in other two laboratories. The 4 laboratories assayed common serum samples as usual procedure of each laboratory.Among different membrane lots, good correlation between each two lot was observed in the five serum protein fractions. There was statistically significant difference among the membrane lots, however, it might be only due to good reproducibility and statistics. The bias was below clinical permission limit.Among laboratories, good correlation between each two laboratory was observed in the five serum protein fractions. The bias between laboratories is below permission limit, compared with bias detected from external quality assessment program for SEPARAX-SP.In conclusion, because the bias in membrane lots and laboratories can be ignored for laboratory diagnosis of serum protein fraction test, SELECA-VSP will come onto the market to make up for SEPARAX-SP.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
对新型纤维素醋酸酯膜‘celeka -VSP’的血清蛋白分块检查的评价:多设施合作研究,用于批件间差异和设施间差异
为了用SELECA-VSP代替SEPARAX-SP,我们研究了SELECA-VSP批次和实验室之间膜性能的差异。我们检测了三批SELECA-VSP,分别是T10、T12和T14。蛋白组分检测使用的自动电泳系统有两个实验室为Olympus AES系列,另外两个实验室为JOKOH CTE系列。4个实验室按各自实验室的常规程序检测普通血清样本。在不同的膜批次中,血清蛋白的5个组分在每两个批次之间均有良好的相关性。膜组间差异有统计学意义,但这可能只是由于良好的再现性和统计学。偏倚低于临床许可限制。在各实验室中,5个血清蛋白组分之间存在较好的相关性。与SEPARAX-SP外部质量评估程序检测到的偏差相比,实验室之间的偏差低于许可限制。综上所述,由于血清蛋白组分检测在实验室诊断中可以忽略膜批和实验室的偏倚,SELECA-VSP将会上市以弥补SEPARAX-SP的不足。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Electrophoretic extraction of protein complexes after separation and detection by a combined method of non-denaturing two-dimensional electrophoresis and reversible staining Use of Escherichia coli expression system for analyzing kinase motifs Proteomic analysis of spheroids of rhabdomyosarcoma cells cultured with decellularized muscle extracts Drug screening and kinase activity profiling of a novel patient-derived cell line of clear cell ovarian carcinoma Proteogenomic approach to drug targets in osteosarcomas with different original sites
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1