Spontaneous Mutations at Fingerprint Loci in Clonal Lineages of the Rice Blast Fungus

Bai Chai Wu, Clint W. Magill
{"title":"Spontaneous Mutations at Fingerprint Loci in Clonal Lineages of the Rice Blast Fungus","authors":"Bai Chai Wu,&nbsp;Clint W. Magill","doi":"10.1006/emyc.1995.1010","DOIUrl":null,"url":null,"abstract":"<div><p>Wu, B.C., and Magill, C. W. 1995. Spontaneous mutations at fingerprint loci in clonal lineages of the rice blast fungus. <em>Experimental Mycology</em> 19, 86-90. When successive asexual generations of monospore-derived cultures from the Tm5 strain of <em>Magnaporthe grisea</em> were subjected to DNA fingerprint analysis with an anonymous genomic DNA probe, gain or loss of hybridizing fragments was common. Three of 24 isolates examined in the first asexual generation gained a <em>Bam</em>Hl fragment. In the next generation six cultures derived from two of these isolates all gained or lost up to three fragments. By comparison, only 1 of 28 isolates from a different culture showed a fingerprint band change, which was the loss of one fragment in the second generation. Where tested, changes in one isolate were also present in its progeny, and since 28 fragments could be scored in the original cultures, the overall patterns remained distinct. However, at the rate of change seen in the Tm5 isolates, fingerprint patterns have the potential to diverge rapidly.</p></div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"19 1","pages":"Pages 86-90"},"PeriodicalIF":0.0000,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1995.1010","citationCount":"14","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental Mycology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0147597585710109","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 14

Abstract

Wu, B.C., and Magill, C. W. 1995. Spontaneous mutations at fingerprint loci in clonal lineages of the rice blast fungus. Experimental Mycology 19, 86-90. When successive asexual generations of monospore-derived cultures from the Tm5 strain of Magnaporthe grisea were subjected to DNA fingerprint analysis with an anonymous genomic DNA probe, gain or loss of hybridizing fragments was common. Three of 24 isolates examined in the first asexual generation gained a BamHl fragment. In the next generation six cultures derived from two of these isolates all gained or lost up to three fragments. By comparison, only 1 of 28 isolates from a different culture showed a fingerprint band change, which was the loss of one fragment in the second generation. Where tested, changes in one isolate were also present in its progeny, and since 28 fragments could be scored in the original cultures, the overall patterns remained distinct. However, at the rate of change seen in the Tm5 isolates, fingerprint patterns have the potential to diverge rapidly.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
稻瘟病菌无性系指纹位点的自发突变
Wu, b.c.和Magill, c.w. 1995。稻瘟病菌无性系指纹位点的自发突变。实验真菌学,19,86-90。用匿名基因组DNA探针对稻瘟病菌Tm5株单孢子培养物的连续无性代进行DNA指纹分析,杂交片段的增加或丢失是常见的。在第一代无性繁殖的24个分离株中,有3个获得了BamHl片段。在下一代中,从这些分离株中的两个衍生出的六个培养株都获得或失去了多达三个片段。相比之下,来自不同培养的28株分离物中只有1株出现指纹带变化,即在第二代中丢失了1个片段。在测试中,一个分离物的变化也出现在其后代中,并且由于在原始培养中可以获得28个片段,因此总体模式仍然不同。然而,从Tm5分离株的变化速度来看,指纹模式有可能迅速分化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Biosynthetic Pathways of Glycerol Accumulation under Salt Stress in Aspergillus nidulans Involvement of Candida albicans Cell Wall Proteins in the Adherence of Blastospores to Human Buccal Epithelial Cells Extraction and Characterization of the Insecticidal Toxin Hirsutellin A Produced by Hirsutella thompsonii var. thompsonii Superoxide Dismutase: A Differentiation Protein Expressed in Uromyces Germlings during Early Appressorium Development A Unique Repeated DNA Sequence in the Cyclosporin-Producing Strain of Tolypocladium inflatum (ATCC 34921)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1