Reducing data acquisition for fast Structured Illumination Microscopy using Compressed Sensing

Abstract

In this work, we introduce an original strategy to apply the Compressed Sensing (CS) framework to a super-resolution Structured Illumination Microscopy (SIM) technique. We first define a framework for direct domain CS, that exploits the sparsity of fluorescence microscopy images in the Fourier domain. We then propose an application of this method to a fast 4-images SIM technique, which allows to reconstruct super-resolved fluorescence microscopy images using only 25% of the camera pixels for each acquisition.