METHODOLOGICAL ASPECTS OF LABORATORY SELECTION OF MEANS FOR SOIL DISINFECTION

Abstract

Soil, bottom sediments, and sewage sludge are the main sources of infection of humans and animals with helminth eggs, and protozoan cysts and oocysts. For the disinfection of environmental objects, chemical disinfectants are successfully used. To identify the most effective disinfection means for soil and environmental objects, laboratory studies of the ovicidal activity of disinfectants are carried out on soil samples. Currently, laboratory practice has no standard methods available for determining the ovicidal efficacy of disinfectants in the soil. A device is offered for the experimental selection of disinfectants for disinfecting soil, geologic material, sewage sludge or bottom sediments contaminated with helminth eggs, and protozoan cysts and oocysts. The laboratory device is a three-segment steel column in the form of a cylinder with supports provided at the base, and a cover with a foam outlet and a filler neck located on the upper part, and a storage tank for collecting the spent disinfectant solution located in the lower part. The cylinder is filled with soil segment by segment, and microcontainers with bioagent are placed at the bottom of each segment. The soil is poured with a disinfectant; and the microcontainers are removed from the soil after various exposure periods to determine the ovicidal activity of the disinfectant.