CHANGES IN SOME VALUES ON THE HEMOGRAM IN THE CHRONIC PHASE OF OPISTHORCHIASIS IN THE EXPERIMENT

Abstract

Deviations of hemogram values in distant periods of invasion in opisthorchiasis are poorly studied, they are only usually studied in case of serious complications of the disease. An experiment was carried out on sexually mature male rodents (rabbits). The invasive dose for each animal in the observation group was 50 metacercariae of Opisthorchis felineus (n=10). Clinically healthy male animals of the same age and sex (n=10) were the control group. The leukogram was studied after 30 months to assess the condition of the animals. Capillary blood smears were fixed and stained by May-Grunwald and Romanowsky-Giemsa methods and examined by light immersion microscopy manually using the meander method. Statistical data processing was performed using the Microsoft Excel application package by calculating the arithmetic mean with the standard error of the mean. Analysis of intergroup data was performed using nonparametric Mann-Whitney U-criterion for unrelated populations with non-normal distribution with p ≤ 0.05. In an intergroup comparison of the data obtained, the number of lymphocytes in the observation group was 1.35 less than the control; stab neutrophils were 5.8 less than the control; the differences were statistically significant. The number of segmented neutrophils in the observation group was 1.38 greater than the control (differences were statistically insignificant), eosinophils were 9.6 greater and basophils were 12.3 greater, and differences were statistically significant. It was found that lymphopenia, stab neutrophils, segmented neutrophils, eosinophilia and basophilia were observed in the chronic phase of infection in the rabbits after 30 months.