Human placental alkaline phosphatase covalently immobilised on a cross-flow microfiltration polyvinylidene difluoride membrane Part II. Activity vs. [substrate] kinetic behaviour
M.G. Roig , J.F. Bello , S. Rodriguez , J.M. Cachaza , J.F. Kennedy
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引用次数: 1
Abstract
Human placental alkaline phosphatase was chemically immobilized on a hydrophilic cross-flow microfiltration membrane made of polyvinylidene difluoride (PVDF) chemically labelled with 1-carbonylimidazole groups and characterized physicochemically (in Part I, J. Mol. Catal. (Vol. 93, 85–104)) The intrinsic kinetic behaviour (rate vs. substrate concentration) in the absence of diffusional constraints was analysed graphically and numerically, by non-linear regression and use of the F statistical test for model discrimination, postulating a minimum rational rate equation of 2:2 degree in substrate concentration. According to the results found, a mechanism for the catalytic kinetic action has been postulated.
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