Characterization of mitochondrial DNA polymorphisms in the Han population in Liaoning Province, Northeast China

IF 1.1 4区生物学 Q4 GENETICS & HEREDITY Mitochondrial Dna Part a Pub Date : 2018-02-17 DOI:10.1080/24701394.2016.1275597

Feng-ling Xu, Jun Yao, M. Ding, Zhang-sen Shi, Xue Wu, Jing-jing Zhang, Bao-jie Wang

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引用次数: 5

Abstract

Abstract This study characterized the genetic variations of mitochondrial DNA (mtDNA) to elucidate the maternal genetic structure of Liaoning Han Chinese. A total of 317 blood samples of unrelated individuals were collected for analysis in Liaoning Province. The mtDNA samples were analyzed using two distinct methods: sequencing of the hypervariable sequences I and II (HVSI and HVSII), and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the coding region. The results indicated a high gene diversity value (0.9997 ± 0.0003), a high polymorphism information content (0.99668) and a random match probability (0.00332). These samples were classified into 305 haplotypes, with 9 shared haplotypes. The most common haplogroup was D4 (12.93%). The principal component analysis map, the phylogenetic tree map, and the genetic distance matrix all indicated that the genetic distance of the Liaoning Han population from the Tibetan group was distant, whereas that from the Miao group was relatively close.

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辽宁省汉族人群线粒体DNA多态性特征分析

摘要本研究通过对辽宁汉族线粒体DNA (mtDNA)遗传变异的分析，阐明辽宁汉族母系遗传结构。在辽宁省共收集了317份无血缘关系个体的血液样本进行分析。采用两种不同的方法对mtDNA样本进行分析:对高变序列I和II (HVSI和HVSII)进行测序，对编码区进行聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析。基因多样性值(0.9997±0.0003)较高，多态性信息含量(0.99668)较高，随机匹配概率(0.00332)较高。这些样本被分类为305个单倍型，其中9个共有单倍型。最常见的单倍群为D4(12.93%)。主成分分析图谱、系统发育树图谱和遗传距离矩阵均表明辽宁汉族群体与藏族群体的遗传距离较远，而与苗族群体的遗传距离较近。

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来源期刊

Mitochondrial Dna Part a Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： Mitochondrial DNA Part A publishes original high-quality manuscripts on physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism, and/or interactions. Manuscripts on cytosolic and extracellular mtDNA, and on dysfunction caused by alterations in mtDNA integrity as well as methodological papers detailing novel approaches for mtDNA manipulation in vitro and in vivo are welcome. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The Journal also considers manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences, as well as papers that discuss the utility of mitochondrial DNA information in medical studies and in human evolutionary biology.