Effects of processing on bacteriological, proximate, hydrocarbon content and organoleptic indices of Scomber scombrus

I. I. James, C. Etuk, M. Ben, R. Okon, A. Essien, A. Asuquo, E. A. Umoren
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Abstract

Effects of boiling, smoking and frying on the organoleptic quality, bacteriological count, proximate composition and total hydrocarbon contents of  Scomber scombrus fish sold in Ikot Ekpene Metropolis were investigated. Fillets of fresh S. scombrus were made, and processed by smoking, boiling and  frying methods. Portions of the fish sample were aliquoted, and used to carry out sensory evaluation, plating aliquots of diluents onto nutrient agar  plates (for total viable count (TVC), and on Manitol Salt agar (for total staphylococcal count (TSC). Standard methods were used to determine moisture,  ash, total lipids and crude protein contents. Total hydrocarbon content was determined by toluene extraction and measurement of absorbance of filtrate  spectrophotometrically. Sensory quality obtained using smoke-drying and frying methods scored significantly higher than that of boiling and the control  (p<0.05). TVC of samples were lower than in the control fish sample after processing, increasing over the 4-day period, although negligible when  compared to the control. TVC were lowest in samples processed by frying. TSC decreased in all fish samples after processing, except in the control fish  sample. Fried Scomber scombrus samples also showed no trace of S. aureus. Moisture, lipid, crude protein and ash contents of control S. scombrus were  68.78±1.02, 2.03±0.11, 20.14±0.06 and 0.09±0.32 respectively. The changes in moisture, lipid, crude protein and ash contents were statistically significant  (P<0.05) in processed fish samples. Total hydrocarbon content also increased in processed fish; 2.914±0.005 for smoke-dried, 2.168±0.001 for fried and  1.538±0.009 for boiled, as against 1.412±0.011 in the control sample. Overall bacteriological quality indices, the proximate content and total hydrocarbon  contents of samples demonstrated frying and smoking as preferable processing methods for Scomber scombrus.    
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加工对小檗细菌学、近似值、烃类含量和感官指标的影响
研究了水煮、烟熏和煎炸对Ikot Ekpene Metropolis市售Scomber scombrus鱼感官品质、细菌数量、近似成分和总烃含量的影响。采用烟熏法、水煮法和煎炸法制备了鲜黑豆肉片。将部分鱼样品进行aliquog,并用于感官评估,将稀释剂等量涂在营养琼脂板上(用于总活菌计数(TVC)),并涂在甘露醇盐琼脂上(用于总葡萄球菌计数(TSC))。采用标准方法测定水分、灰分、总脂和粗蛋白质含量。采用甲苯萃取法和分光光度法测定总烃含量。烟熏烘法和煎炸法的感官品质评分显著高于水煮法和对照组(p<0.05)。处理后样品的TVC低于对照鱼样品,在4天内有所增加,但与对照相比可以忽略不计。油炸加工的样品中TVC含量最低。除对照鱼样本外,处理后所有鱼样本的TSC均下降。炸后的鸡花样品也没有发现金黄色葡萄球菌的痕迹。对照云杉水分、脂肪、粗蛋白质和灰分含量分别为68.78±1.02、2.03±0.11、20.14±0.06和0.09±0.32。加工鱼样的水分、脂肪、粗蛋白质和灰分含量变化均有统计学意义(P<0.05)。加工鱼的总烃含量也有所增加;烟熏样品为2.914±0.005,油炸样品为2.168±0.001,水煮样品为1.538±0.009,对照样品为1.412±0.011。综合细菌学质量指标、样品的近似烃含量和总烃含量表明煎炸和烟熏是较好的处理方法。
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