lncRNA ELFN1-AS1 predicts poor prognosis and promotes tumor progression of non-small cell lung cancer by sponging miR-497.

IF 1.9 4区医学 Q3 ONCOLOGY Cancer Biomarkers Pub Date : 2022-01-01 DOI:10.3233/CBM-210393

Bin Yang, Shuai Miao

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Abstract

Background: More novel biomarkers need to be discovered to improve the therapeutic efficiency of non-small cell lung cancer (NSCLC). lncRNA ELFN1-AS1 (ELFN1-AS1) was proved to play crucial roles in numerous diseases, its intention in NSCLC remains unclear.

Objective: This study aimed to investigate the function of ELFN1-AS1 and its potential mechanism in NSCLC development.

Methods: A total of 117 NSCLC patients were recruited and provided paired NSCLC tissues and normal tissues. The expression of ELFN1-AS1 was analyzed by PCR. The biological function of ELFN1-AS1 was estimated by CCK8 and Transwell assay. Additionally, the potential mechanism underlying the function of ELFN1-AS1 was explored by the dual-luciferase reporter assay and western blotting.

Results: The significant upregulation of ELFN1-AS1 was found in NSCLC tissues and cells, which was closely associated with the TNM stage, lymph node metastasis status, and overall survival of patients. The knockdown of ELFN1-AS1 was found to inhibit the cellular processes and EMT of NSCLC. Moreover, ELFN1-AS1 was found to serve as a sponge to binding with miR-497, and CCNE1 was demonstrated to be the downstream target of miR-497, which was speculated as the potential mechanism underlying the function of ELFN1-AS1.

Conclusions: ELFN1-AS1 acts as an independent prognostic biomarker and tumor promoter of NSCLC by sponging miR-497/CCNE1 axis.

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lncRNA ELFN1-AS1通过海绵化miR-497预测非小细胞肺癌的不良预后并促进肿瘤进展。

背景：需要发现更多新的生物标志物来提高非小细胞肺癌（NSCLC）的治疗效率。lncRNA ELFN1-AS1 （ELFN1-AS1）已被证实在许多疾病中发挥重要作用，但其在NSCLC中的作用尚不清楚。目的：探讨ELFN1-AS1基因在非小细胞肺癌发生发展中的作用及其潜在机制。方法：招募117例非小细胞肺癌患者，提供配对的非小细胞肺癌组织和正常组织。采用PCR方法分析ELFN1-AS1的表达情况。采用CCK8和Transwell法测定ELFN1-AS1的生物学功能。此外，通过双荧光素酶报告试验和western blotting研究了ELFN1-AS1功能的潜在机制。结果：ELFN1-AS1在NSCLC组织细胞中表达显著上调，与患者TNM分期、淋巴结转移情况及总生存期密切相关。ELFN1-AS1基因敲低可抑制非小细胞肺癌的细胞过程和EMT。此外，ELFN1-AS1被发现作为海绵与miR-497结合，CCNE1被证明是miR-497的下游靶点，推测这是ELFN1-AS1功能的潜在机制。结论：ELFN1-AS1通过海绵化miR-497/CCNE1轴作为非小细胞肺癌的独立预后生物标志物和肿瘤启动子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Biomarkers ONCOLOGY-

CiteScore

5.20

自引率

3.20%

发文量

195

审稿时长

3 months

期刊介绍： Concentrating on molecular biomarkers in cancer research, Cancer Biomarkers publishes original research findings (and reviews solicited by the editor) on the subject of the identification of markers associated with the disease processes whether or not they are an integral part of the pathological lesion. The disease markers may include, but are not limited to, genomic, epigenomic, proteomics, cellular and morphologic, and genetic factors predisposing to the disease or indicating the occurrence of the disease. Manuscripts on these factors or biomarkers, either in altered forms, abnormal concentrations or with abnormal tissue distribution leading to disease causation will be accepted.