Effect of certain additives on the quality of boar semen during preservation at 15°C and 5°C

Tejoswita Saikia, K. Ahmed, P. M. Barua, B. Deka, N. Ahmed
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引用次数: 1

Abstract

The study was aimed to evaluate the effect of four additives viz. KMnO4, Vitamin E, Butylatedhydroxytoluene and trehalose on quality of boar semen during preservation at 15°C and 5°C up to 96 hours. 24 ejaculates were utilized for the study. Sperm motility was significantly higher with BHT irrespective of preservation temperature and period. However, the mean sperm motility was significantly higher (P<0.05) in semen preserved at 15°C than 5°C. Live sperm was significantly (P<0.05) lower in Trehalose than KMNO4, Vitamin E and BHT. The mean percentage of live intact acrosome irrespective of temperature and preservation period was significantly higher (P<0.05) with BHT than others additives. Semen in Modena extender with BHT had significantly higher (P<0.05) percentage of HOST-reacted sperm at different preservation periods irrespective of different temperature. In conclusion, Butylatedhydroxyltoluene (BHT) was found to be superior to KMNO4, Vitamin E and Trehalose for preservation of Hampshire boar semen.
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15℃和5℃条件下某些添加剂对猪精液品质的影响
本试验旨在评价4种添加剂KMnO4、维生素E、丁基羟基甲苯和海藻糖在15℃和5℃条件下保存96 h时对公猪精液质量的影响。研究中使用了24种射精。无论保存温度和时间如何,BHT均显著提高了精子活力。然而,15°C保存的精液平均精子活力显著高于5°C保存的精液(P<0.05)。海藻糖对活精子的影响显著(P<0.05)低于KMNO4、维生素E和BHT。添加BHT显著高于其他添加剂(P<0.05),且不受温度和保存期影响。添加BHT的摩德纳鱼在不同保存时间的精液中,无论温度如何,宿主反应精子的比例都显著提高(P<0.05)。综上所述,丁基羟基甲苯(BHT)的保存效果优于KMNO4、维生素E和海藻糖。
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