Yogurt containing Lactobacillus gasseri PA-3 alleviates increases in serum uric acid concentration induced by purine ingestion: a randomized, double-blind, placebo-controlled study

Abstract

Objective: Ingestion of yogurt containing Lactobacillus gasseri PA-3(PA-3, Accession No : NITE BP-224) (PA-3Y)has been shown to reduce serum uric acid(SUA)levels by interfering with the intestinal absorption of food-derived purines in animal studies. To confirm this mechanism in humans, the ability of PA-3 intake to alleviate purine ingestion-induced increases in SUA levels was analyzed. Research Methods and Procedures: In this randomized, double-blind, placebo-controlled crossover study, 16 healthy adults were randomized to groups ingesting of 112 g of PA-3Y or yogurt without PA-3(control yogurt)in addition to standardized meals for 3 days. Purine-loading tests, in which subjects ingested 112 g of PA-3Y or control yogurt followed immediately by 498 mg of a mixture of purine nucleotides, were performed on the fourth day of each test period. Blood and urine samples were collected before and after the purine-loading tests. Results : The increase in the SUA concentration from the baseline was significantly lower following the ingestion of PA-3Y than of control yogurt alone, especially at 30(P=0.033)and 60(P=0.028)minutes. In addition, the area under the curve for the increase in the SUA concentration from the baseline to 150 minutes was also significantly lower (P=0.041)in the PA-3Y than in the control yogurt group. However, urinary and fractional excretions of uric acid were not different between the two groups. Conclusion : The ingestion of PA-3 before purine intake alleviates the increase in SUA levels, probably by reducing purine absorption in the intestine, and not by enhancing urinary excretion of uric acid. Introduction Hyperuricemia is defined as a serum uric acid (SUA)concentration over 7.0 mg dL1. Persistent hyperuricemia can lead to the deposition of urate crystals in the joints and kidneys, resulting in acute arthritis or kidney injury such as nephrolithiasis and nephropathy13). Hyperuricemia has also been associated Masafumi Kurajoh1, 2) Yuji Moriwaki1) Hidenori Koyama1) Hiroshi Tsuboi3) Hirotaka Matsuda3) Naruomi Yamada3) Chizuru Saito3) Hiroshi Kano3) Yukio Asami3) Tetsuya Yamamoto1) Yogurt containing Lactobacillus gasseri PA-3 alleviates increases in serum uric acid concentration induced by purine ingestion: a randomized, double-blind, placebo-controlled study 受付:2017年11月10日,受理:2018年3月2日 1) Division of Diabetes, Endocrinology and Metabolism, Department of Internal Medicine, Hyogo College of Medicine 2) Department of Metabolism, Endocrinology, and Molecular Medicine, Osaka City University Graduate School of Medicine 3) Food Science Research Laboratories, Meiji Co., LTD.