Molecular Characterization and Expression Profiling of ENOX2 Gene in Response to Heat Stress in Goats

Abstract

NADPH oxidase (NOX) proteins are membrane-associated, multi-unit enzymes that catalyze the reduction of oxygen using NADPH as an electron donor. The present study was carried out to analyse expression profile of ENOX2 gene in heat stress susceptible (HSS) and heat stress tolerant (HST) individuals in response to heat stress. The genomic DNA was isolated from blood. The total RNA was isolated from different tissues such as liver, spleen and kidney. High Resolution Melting (HRM) genotyping exhibited two different genotypes in four goat breeds. The relative expression pattern of ENOX2 gene in liver, spleen and kidney showed 7.40 10.01 and 3.92 fold higher expression than control. Barbari, Jamunapari, Jakhrana and Sirohi exhibited 8.11, 10.97, 5.65 and 5.87 higher fold expression than control. Heat stress-susceptible (HSS) and heat stress-tolerant (HST) individuals exhibited 12.45 and 5.41 fold ENOX2 gene expressions than control in response to long term heat stress.