Effect of agitation on extraction of phenolic compounds from ripe pericarp of Wodyetia bifurcata

Thanapat Sakdasukon, S. Thanachasai
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Abstract

In this study, the ripe pericarp extracts of Wodyetia bifurcata were prepared by solvent extraction techniques with and without agitation. The influence of agitation was examined on the extraction yield, total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity of the ripe pericarp extracts of Wodyetia bifurcata. Antioxidant capacity of extracts was evaluated by measuring the scavenging effect on 2, 2’-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) radicals. Compared to solvent extraction without agitation, solvent extraction with agitation significantly (p < 0.05) increased the extraction yield, TPC, TFC and antioxidant capacities (ABTS and DPPH) of the ripe pericarp extracts of Wodyetia bifurcata. In addition, higher agitation speed resulted in higher extraction yield, TPC, TFC and antioxidant activities (ABTS and DPPH) of the ripe pericarp extracts of Wodyetia bifurcata.
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搅拌对双叶木参成熟果皮中酚类化合物提取的影响
本研究采用溶剂萃取法和无搅拌萃取法分别制备了双曲木耳成熟果皮提取物。研究了搅拌条件对双叶树成熟果皮提取物提取率、总酚含量(TPC)、总黄酮含量(TFC)和抗氧化活性的影响。通过测定提取物对2,2′-氮唑(3-乙基苯并噻唑-6-磺酸)(ABTS)和2,2′-二苯基-1-吡啶肼(DPPH)自由基的清除作用,评价其抗氧化能力。与不搅拌的溶剂提取相比,有搅拌的溶剂提取显著(p < 0.05)提高了双歧木叶成熟果皮提取物的提取率、TPC、TFC和抗氧化能力(ABTS和DPPH)。此外,搅拌速度越快,双叶树成熟果皮提取物的提取率、TPC、TFC和抗氧化活性(ABTS和DPPH)越高。
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