Isolation and Selection of Functional Microbes for Eco-friendly Turfgrass Management in Golf Course from Livestock Manure Compost

Weed&Turfgrass Science Pub Date : 2017-06-30 DOI:10.5660/WTS.2017.6.2.157

Je-Yong Jeong, Young-sun Kim, S. Cho, Geung-Joo Lee

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引用次数: 2

Abstract

. Functional microorganisms decompose various organic matter by enzyme activity and suppress plant disease caused by pathogen. This study was conducted to isolate and select functional microorganisms with protein or carbohydrate degradation activities and antagonistic activity against turfgrass fungal pathogens for eco-friendly turfgrass management in golf course from compost containing livestock manure of poultry or swine. Totally 68 isolates collected from livestock manure compost strains were isolated and tested for their activities of amylase, protease and lipase and antagonistic activities against Rhizoctonia solani AG2-2 , R. solani AG1-1 , and Sclerotinia homoeocarpa . Among the isolates, 34 strains were selected as functional microbes showing higher activities of amylase and protease. Three isolates of ASC-14, ASC-18, and ASC-35 among the 34 strains were selected as antifungal bacterial strains repressing the above 3 turfgrass fungal pathogens. Analysis results of 16s rRNA gene sequence and phylogenic cluster indicated that ASC-14 and ASC-18 belonged to Bacillus amyloliquefaciens , while ASC-35 was B. subtilis , respectively.

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畜禽粪便堆肥中高尔夫球场草坪生态管理功能微生物的分离与筛选

．功能微生物通过酶活性分解各种有机物，抑制病原菌引起的植物病害。本研究旨在从含家禽或猪粪的堆肥中分离和筛选具有蛋白质或碳水化合物降解活性和对草坪草真菌病原体拮抗活性的功能性微生物，用于高尔夫球场草坪草生态管理。对从畜禽粪便堆肥菌株中分离得到的68株分离菌进行了淀粉酶、蛋白酶和脂肪酶活性测定以及对茄枯丝核菌AG2-2、茄枯丝核菌AG1-1和同质核菌核菌的拮抗活性测定。筛选出34株淀粉酶和蛋白酶活性较高的功能菌。从34株病原菌中筛选出3株ASC-14、ASC-18和ASC-35作为抑制上述3种草坪草真菌病原菌的抗真菌菌株。16s rRNA基因序列和系统发育聚类分析结果表明，ASC-14和ASC-18分别属于解淀粉芽孢杆菌，ASC-35分别属于枯草芽孢杆菌。

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Weed&Turfgrass Science

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