{"title":"ANTI-PROLIFERATIVE ACTIVITIES OF THE AQUEOUS ROOT EXTRACT OF DIANTHUS THUNBERGII SS HOOPER (CARYOPHYLLACEAE)","authors":"A. Akinrinde, M. Venter, T. Koekemoer, G. Bradley","doi":"10.21010/AJTCAM.V15I4.5","DOIUrl":null,"url":null,"abstract":"Background: The roots of Dianthus thunbergii SS Hooper are used traditionally in South Africa for the treatment of \ndiabetes, wounds, colic, chest complaints and cancer. This study was aimed at investigating the potential anti-proliferative \nactivities of the D. thunbergii in mammalian cancer cell lines. \nMaterials and Methods: Aqueous and ethanol extracts of D. thunbergii were tested in vitro on two cancer cell lines: \nhuman hepato-cellular carcinoma (HepG2) cells and murine insulinoma (INS-1) cells using the 3-(4,5-Dimethylthiazol-2- \nyl) 2,5-diphenyltetrazolium bromide (MTT) and crystal violet cell viability assays, as well as live-cell fluorescence imaging \nmicroscopy. A tentative profiling of the aqueous extract was also carried out using liquid chromatography-mass \nspectrometry (LC-MS). \nResults: The aqueous extract (50-200μg/ml) exhibited significant (p","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"16 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"African Journal of Traditional, Complementary and Alternative Medicines","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21010/AJTCAM.V15I4.5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Background: The roots of Dianthus thunbergii SS Hooper are used traditionally in South Africa for the treatment of
diabetes, wounds, colic, chest complaints and cancer. This study was aimed at investigating the potential anti-proliferative
activities of the D. thunbergii in mammalian cancer cell lines.
Materials and Methods: Aqueous and ethanol extracts of D. thunbergii were tested in vitro on two cancer cell lines:
human hepato-cellular carcinoma (HepG2) cells and murine insulinoma (INS-1) cells using the 3-(4,5-Dimethylthiazol-2-
yl) 2,5-diphenyltetrazolium bromide (MTT) and crystal violet cell viability assays, as well as live-cell fluorescence imaging
microscopy. A tentative profiling of the aqueous extract was also carried out using liquid chromatography-mass
spectrometry (LC-MS).
Results: The aqueous extract (50-200μg/ml) exhibited significant (p
期刊介绍：
The “African Journal of Traditional, Complementary and Alternative Medicines (AJTCAM)” is a peer-reviewed, multidisciplinary, international, scientific Open Access Journal that provides publication of articles on phytomedicines, ethnomedicines and veterinary ethnomedicines. The journal is published by a Non-Governmental Organization (NGO) known as “African Traditional Herbal Medicine Supporters Initiative (ATHMSI)”. The Journal welcomes submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published approximately two-to-three months after acceptance
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