Prognostic significance of serum miR-18a-5p in severe COVID-19 Egyptian patients.

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-11-13 DOI:10.1186/s43141-023-00565-y
Riham Abdel-Hamid Haroun, Waleed H Osman, Asmaa M Eessa
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Abstract

Background: The identification of miRNAs as well as characterization of miRNA-mRNA interactions in SARS-CoV-2 infection is important to understand their role in disease pathogenesis. Therefore the aim of the present study was to measure the expression levels of hsa-mir-18a-5p in the sera of severe COVID-19 Egyptian patients admitted to ICU to investigate its roles in the pathogenesis and severity of COVID-19 disease.

Methods: A total of 180 unvaccinated severe COVID-19 patients were enrolled in our study. Besides the routine laboratory work, the expression level of hsa-mir-18a-5p was done using reverse transcription quantitative real-time PCR (RTqPCR) technique. Also, target genes of hsa-mir-18a-5p were explored by using online bioinformatics databases.

Results: The expression level of hsa-mir-18a-5p decreased in nonsurvival severe COVID-19 patients (0.38 ± 0.26) when compared to the survival ones (0.84 ± 0.23). While as a prognostic tool for the prediction of bad prognosis and mortality among severe COVID-19 patients, our results showed that the serum hsa-mir-18a-5p expression level is a good sensitive and specific marker. By using bioinformatics tools, our results revealed that the decreased hsa-mir-18a-5p expression level may have a crucial role in COVID-19 pathogenesis and severity through decreased immunological responses (interpreted as lymphopenia) or increased inflammation (interpreted as increased serum levels of IL-6, CRP, LDH).

Conclusion: Taken together, the decreased expression level of hsa-mir-18a-5p could be a bad prognostic marker and therapeutic overexpression of hsa-mir-18a-5p could be a novel approach in the treatment of COVID-19 disease.

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血清miR-18a-5p在重症COVID-19埃及患者中的预后意义
背景:在SARS-CoV-2感染中鉴定mirna以及表征miRNA-mRNA相互作用对了解其在疾病发病机制中的作用具有重要意义。因此,本研究的目的是测量hsa-mir-18a-5p在ICU入院的埃及重症COVID-19患者血清中的表达水平,探讨其在COVID-19疾病发病机制和严重程度中的作用。方法:共纳入180例未接种疫苗的COVID-19重症患者。在常规实验室工作的基础上,采用逆转录实时定量PCR (RTqPCR)技术检测hsa-mir-18a-5p的表达水平。此外,利用在线生物信息学数据库探索hsa-mir-18a-5p的靶基因。结果:非生存期重症患者hsa-mir-18a-5p表达水平(0.38±0.26)低于生存期患者(0.84±0.23)。我们的研究结果表明,血清hsa-mir-18a-5p表达水平是一种敏感性和特异性较好的标志物,可作为预测COVID-19重症患者不良预后和死亡率的预后工具。通过使用生物信息学工具,我们的研究结果显示,hsa-mir-18a-5p表达水平的降低可能通过降低免疫反应(解释为淋巴细胞减少)或增加炎症(解释为血清IL-6, CRP, LDH水平升高)在COVID-19的发病机制和严重程度中起关键作用。结论:综上所述,hsa-mir-18a-5p表达水平降低可能是不良预后标志物,治疗性过表达hsa-mir-18a-5p可能是治疗COVID-19疾病的新途径。
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