A Simple Plate Method for the Screening and Detection of Fucoidanases

Artem S. Silchenko , Huynh Hoang Nhu Khanh , Cao Thi Thuy Hang , Valeriya V. Kurilenko , Alexander M. Zakharenko , Anastasiya O. Zueva , Bui Minh Ly , Mikhail I. Kusaykin
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引用次数: 5

Abstract

A novel method was developed for the screening and detection of fucoidanase activity in bacterial colonies using fucoidan–agarose plates and staining with hexadecyltrimethylammonium bromide (Cetavlon). Colonies with fucoidanase activity were indicated by a transparent halo. The medium containing undegraded fucoidan showed as a milk-colored background, but areas with degraded fucoidan under the colonies were visualized as a transparent halo.

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一种筛选和检测岩藻糖酸酶的简易平板法
建立了一种利用岩藻糖苷-琼脂糖板和十六烷基三甲基溴化铵(Cetavlon)染色法筛选和检测细菌菌落岩藻糖苷酶活性的新方法。具有岩藻糖苷酶活性的菌落用透明光晕表示。含有未降解岩藻糖聚糖的培养基显示为乳色背景,但菌落下含有降解岩藻糖聚糖的区域显示为透明晕。
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