mySpot: a versatile microfocussing station for scanning methods at BESSY II

I. Zizak
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引用次数: 4

Abstract

mySpot is a versatile microfocussing station for scanning methods with resolution down to 1.5 µm, providing a combination of methods which can be performed simultaneously at the same sample position. It is especially designed (but not limited to) for the study of hierarchically structured biological samples. Structural information from different length scales (XRD - crystalline, SAXS - nanometer, Video-microscope - micrometer, sample translation - milimeter) can be combined with chemical information (XRF-mapping, EXAFS, XANES) and molecular information (Raman) providing unique insight into the mutual dependencies of different parameters. The beamline provides focal spot of about 500x50µm, which can be refocused at the sample using capillary optics. Capillary optics is used for two main reasons: 1) the focal spot does not depend on the energy which makes EXAFS and XANES measurements very simple, and 2) the optics is positioned very close to the sample which improves the stability of the focus at the sample. This allows the focal spot of 1.5 µm in 2D scans, as well as very parallel beam for scattering experiments down to 10µm, or volume element of 20µm diameter for 3D XRF mapping.
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mySpot: BESSY II扫描方法的多功能微聚焦站
mySpot是一个多功能的微聚焦站,分辨率低至1.5 μ m,提供了可以在同一样品位置同时执行的方法组合。它是特别设计的(但不限于)为分层结构的生物样品的研究。来自不同长度尺度的结构信息(XRD -晶体,SAXS -纳米,视频显微镜-微米,样品平移-毫米)可以与化学信息(xrf测绘,EXAFS, XANES)和分子信息(拉曼)相结合,提供对不同参数相互依赖性的独特见解。光束线提供约500x50µm的焦斑,可以使用毛细管光学重新聚焦在样品上。使用毛细管光学有两个主要原因:1)焦点光斑不依赖于能量,这使得EXAFS和XANES的测量非常简单;2)光学定位非常靠近样品,这提高了焦点在样品上的稳定性。这允许在2D扫描中1.5µm的焦点点,以及非常平行的光束散射实验低至10µm,或20µm直径的体积单元用于3D XRF测绘。
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