Varietal variations in biochemical changes during growth and redifferentiation of rice callus cultures

T. Abe, Y. Oka, T. Sasahara
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引用次数: 8

Abstract

Calli initiated from 7 rice varieties belonging to 3 subspecies, indica, japonica, and javanica were cultured for 2 and 14 months on the callus maintenance medium, transferred to the plant regeneration medium, and various organ redifferentiation/plant regeneration abilities were examined. Considerable variations especially in the shoot regeneration were observed among the varieties; four varieties [Tadukan (indica), Nipponbare and Sasanishiki (japonica) and Allorio (javanica)] retained the same redifferentiation abilities, while the remaining 3 varieties [Fujisaka 5 (japonica) and Te-tep and Choukoutou (indica)] showed only very much lowered abilities after 14 months. During the redifferentiation/regeneration period, samples were taken at 4-day intervals up to 36 days and peroxidase and acid phosphatase activities were measured. Activities of these enzymes increased after transfer to the regeneration medium. The patterns of the increase, although the same among varieties within the same subspecies, differed markedly between subspecies. Significant correlations between the redifferentiation/regeneration abilities and the activities of the two enzymes were observed.
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水稻愈伤组织生长和再分化过程中生化变化的品种差异
以籼稻、粳稻和javanica 3个亚种的7个水稻品种愈伤组织为材料,在愈伤组织维持培养基上培养2个月和14个月,然后转移到植株再生培养基上,观察其器官再分化和植株再生能力。不同品种间的茎部再生差异较大;4个品种[多根(籼稻)、日本光和佐三石(粳稻)和Allorio(爪牙)]保持了相同的再分化能力,而其余3个品种[藤坂5号(粳稻)和特特步和秋口(籼稻)]在14个月后的再分化能力仅下降了很多。在再分化/再生期间,每隔4天至36天取样一次,测定过氧化物酶和酸性磷酸酶的活性。转移到再生培养基后,这些酶的活性增加。虽然同一亚种内的不同品种之间的增长模式相同,但亚种之间的增长模式差异显著。再分化/再生能力与这两种酶的活性之间存在显著的相关性。
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