{"title":"Non-human primate models and in vitro liver stage cultures as alternatives in malaria drug development","authors":"Anne-Marie Zeeman, Clemens H.M. Kocken","doi":"10.1016/j.ddmod.2018.03.001","DOIUrl":null,"url":null,"abstract":"<div><p><span>Non-human primates (NHP) have been used extensively in the identification of new antimalarial compounds. Especially the </span><span><em>Plasmodium cynomolgi</em></span>/rhesus monkey model was a very popular substitute to evaluate compounds for <em>P. vivax</em> liver- and blood stages. Nowadays we have replaced, as much as possible, the NHP <em>in vivo</em> testing with <em>in vitro</em> assays. In this review, an overview is given on malaria drug testing in NHP, the development of the <em>in vitro</em> models and especially the <em>in vitro</em> hypnozoite assay, in which compounds can be tested for activity against dormant liver stage parasites. Benefits and limitations of this model will be discussed.</p></div>","PeriodicalId":39774,"journal":{"name":"Drug Discovery Today: Disease Models","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ddmod.2018.03.001","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Drug Discovery Today: Disease Models","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1740675717300403","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
引用次数: 2
Abstract
Non-human primates (NHP) have been used extensively in the identification of new antimalarial compounds. Especially the Plasmodium cynomolgi/rhesus monkey model was a very popular substitute to evaluate compounds for P. vivax liver- and blood stages. Nowadays we have replaced, as much as possible, the NHP in vivo testing with in vitro assays. In this review, an overview is given on malaria drug testing in NHP, the development of the in vitro models and especially the in vitro hypnozoite assay, in which compounds can be tested for activity against dormant liver stage parasites. Benefits and limitations of this model will be discussed.
期刊介绍：
Drug Discovery Today: Disease Models discusses the non-human experimental models through which inference is drawn regarding the molecular aetiology and pathogenesis of human disease. It provides critical analysis and evaluation of which models can genuinely inform the research community about the direct process of human disease, those which may have value in basic toxicology, and those which are simply designed for effective expression and raw characterisation.
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