{"title":"Cloning and sequencing of the gene encoding chitinase ChiA from Xanthomonas sp. strain AK and some properties of ChiA","authors":"Kazuo Sakka , Ryo Kusaka , Akihiro Kawano , Shuichi Karita , Jiraporn Sukhumavasi , Tetsuya Kimura , Kunio Ohmiya","doi":"10.1016/S0922-338X(99)80001-4","DOIUrl":null,"url":null,"abstract":"<div><p>The <em>chiA</em> gene encoding chitinase A was cloned into <em>Escherichia coli</em> from <em>Xanthomonas</em> sp. strain AK and its nucleotide sequence was determined. The structural gene consists of 1788 bp encoding 596 amino acids with a predicted molecular weight of 62,122. The deduced ChiA is a modular enzyme composed of an N-terminal signal peptide and four domains in the following order: a chitin-binding domain, two fibronectin type III domains, and a family 18 catalytic domain. ChiA purified from the recombinant <em>E. coli</em> had temperature and pH optima at 35°C and 4.5, respectively. The <em>K</em><sub>m</sub> and <em>V</em><sub>max</sub> values for colloidal chitin were estimated to be 1.8 mg/ml and 8.7 μmol/min/mg, respectively.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(99)80001-4","citationCount":"19","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fermentation and Bioengineering","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0922338X99800014","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 19
Abstract
The chiA gene encoding chitinase A was cloned into Escherichia coli from Xanthomonas sp. strain AK and its nucleotide sequence was determined. The structural gene consists of 1788 bp encoding 596 amino acids with a predicted molecular weight of 62,122. The deduced ChiA is a modular enzyme composed of an N-terminal signal peptide and four domains in the following order: a chitin-binding domain, two fibronectin type III domains, and a family 18 catalytic domain. ChiA purified from the recombinant E. coli had temperature and pH optima at 35°C and 4.5, respectively. The Km and Vmax values for colloidal chitin were estimated to be 1.8 mg/ml and 8.7 μmol/min/mg, respectively.
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