Novel and simple two-step purification of a full-length rat glucocorticoid-receptor expressed in a baculovirus system

Abstract

We purified the activated recombinant glucicorticoid receptor (GR) overexpressed in insect cells by sequential chromatographies using Mono Q and Mono S columns. This procedure was based upon a new finding that the activated GR binds both to a Mono Q column and to a Mono S column at the same pH (pH 8.4). The entire chromatographies took about 3 h and GR represented 97% of the purified protein sample. The purified GR was able to bind specifically to a DNA fragment containing the glucocorticoid response element. This purification protocol will be applicable to the purification of native GR, point-mutated recombinant GR and other nuclear receptors.