In-vitro Evaluation of Actinobacteria for its Potential in Bio-control of Fungal Plant Pathogens

Zothanpuia, W. Carrie, V. V. Leo, A. K. Passari, R. Lalmuanpuii, B. Singh
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Abstract

Infections caused by fungal plant pathogens are recently recognized as a threat to food security worldwide and its control strategies need to be taken care where naturally synthesized fungicides such as those obtained from actinobacteria are becoming an area of great interest. A total of 68 isolates of actinobacteria were evaluated for their antagonistic potential against four fungal plant pathogens viz., Fusarium oxysporum CABI-293942, Fusarium udum MTCC-2755, Fusarium proliferatum MTCC-286 and Fusarium graminearum MTCC-1893 by dual culture assay. It was found that 83.8% of the isolates showed inhibitory activity against at least one of the tested plant pathogens with the percentage of inhibition ranging from 20–87.2. Thirteen Streptomyces isolates and one Nocardiposis isolate exhibited inhibition activity against all the tested pathogens. Overall, this study gives a basic understanding of the potential aspect of freshwater sediments derived actinobacteria against fungal phytopathogens.
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放线菌对植物真菌病原菌生物防治潜力的体外评价
真菌植物病原体引起的感染最近被认为是对全球粮食安全的威胁,需要注意其控制策略,其中自然合成的杀菌剂(如从放线菌中获得的杀菌剂)正成为一个非常感兴趣的领域。采用双培养法测定了68株放线菌对4种植物真菌病原菌(镰刀菌CABI-293942、镰刀菌MTCC-2755、增殖镰刀菌MTCC-286和谷物镰刀菌MTCC-1893)的拮抗能力。结果表明,83.8%的菌株对至少一种植物病原菌具有抑制活性,抑制率在20 ~ 87.2之间。13株链霉菌和1株诺卡迪菌对所有病原菌均有抑制活性。总的来说,本研究对淡水沉积物中放线菌对真菌植物病原体的潜在作用有了基本的了解。
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