Spontaneous Cell-Mediated Cytotoxicity (SCMC) Associated with Lymphocytes Negative for Acid α-Naphthyl Acetate Esterase (ANAE) Activity

Zeitschrift für Immunit?tsforschung: Immunobiology Pub Date : 1979-02-01 DOI:10.1016/S0340-904X(79)80017-2

V. Rajvanshi , H.H. Peter , H.J. Avenarius

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引用次数: 8

Abstract

A modified histochemical procedure for nonspecific acid α-naphthyl acetate esterase (ANAE) activity in human lymphocytes was used to identify a subpopulation of E-rosette forming cells. Performing a one hour reaction at pH 6.5 the distinct dot-like staining pattern was almost exclusively observed on high affinity E-rosettes which sedimented readily in a regular Ficoll-Metrizoate gradient. By combining latex phagocytosis with staining for ANAE activity, a clear-cut distinction between mononuclear phagocytes and lymphocytes could be made. An attempt was undertaken to relate the ANAE marker on human lymphocytes to their functional capacity in spontaneous cell-mediated cytotoxicity (SCMC) reactions. Using as targets two allogeneic (K562,IGR3) and a xenogeneic cell line (L1210) it could be clearly demonstrated that high SCMC activity is mediated by ANAE negative mononuclear cells, whereas enrichment for ANAE positive lymphocytes resulted in a loss of SCMC.

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α-醋酸萘酯酶(ANAE)活性阴性淋巴细胞的自发细胞介导细胞毒性(SCMC

采用改进的组织化学方法检测人淋巴细胞中非特异性酸性α-乙酸萘酯酶(ANAE)活性，以鉴定e -玫瑰花结形成细胞亚群。在pH 6.5下进行1小时的反应，明显的点状染色模式几乎只在高亲和力的e -莲座上观察到，这些莲座很容易以规则的ficoll - metrizate梯度沉积。将胶乳吞噬与ANAE活性染色相结合，可以明确区分单核吞噬细胞和淋巴细胞。我们试图将人淋巴细胞上的ANAE标记物与它们在自发细胞介导的细胞毒性(SCMC)反应中的功能能力联系起来。使用两个同种异体(K562,IGR3)和一个异种细胞系(L1210)作为靶点，可以清楚地证明高SCMC活性是由ANAE阴性的单核细胞介导的，而ANAE阳性淋巴细胞的富集导致SCMC的丢失。

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Zeitschrift für Immunit?tsforschung: Immunobiology

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