Evaluation of Relationship Between Lymphocyte DNA Damages and Blood Arsenic Levels in Silver Mining Workers using Alkaline Comet Assay

Esma Söylemez, Z. Kayaalti, D. Kaya-Akyüzlü, E. Tutkun, T. Söylemezoğlu
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引用次数: 2

Abstract

Bu calismadaki amac, mesleki arsenige maruz bireylerde kan arsenik (As) duzeyi ve DNA hasar arasindaki iliskiyi arastirmaktir. Gumus madeni iscilerinden 120 bireyin lenfositlerindeki DNA hasari comet yontemi (tek hucre jel elektroforezi) kullanilarak belirlendi. DNA hasar duzeyleri BAB Bs Comet Assay sistemi ile hesaplandi ve As duzeyleri atomic absorbsiyon spektroskopi (AAS) cihazi ile analiz edildi. Kan As duzeyi ortalamasi 17.03±10.85 μg/L olarak tespit edildi. Kan As ve comet parametreleri (kuyruk yogunlugu, comet uzunlugu ve kuyruk uzunlugu) arasinda istatistiksel olarak anlamli pozitif korelasyon bulundu (sirasiyla; r=0.360, r=0.334, r=0.259; p<0.01). Sonuclarimiz, Turk Eti Maden gumus iscilerinde arsenik maruziyetinin periferik lenfosit DNA hasarina sebep oldugunu gosterdi.
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碱性彗星法评价银矿工人淋巴细胞DNA损伤与血砷水平的关系
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