Pao-Wen Grace Liu , Liang-Ming Whang , Ming-Chieh Yang , Sheng-Shung Cheng
{"title":"Biodegradation of diesel-contaminated soil: A soil column study","authors":"Pao-Wen Grace Liu , Liang-Ming Whang , Ming-Chieh Yang , Sheng-Shung Cheng","doi":"10.1016/j.jcice.2008.03.006","DOIUrl":null,"url":null,"abstract":"<div><p>This study simulated <em>in situ</em> bioremediation for diesel-contaminated soil by a column operation. Several bioremediation approaches were conducted to examine the feasibility. Four lab-scale soil columns were operated specific to the following approaches: nutrient enhancement (NE), bioaugmentation (BA), biostimulation (BS), and sterilized soil (SS). Within 330 days of operation, the residual total petroleum hydrocarbon-diesel (TPH<sub>d</sub>) was degraded from 10,290<!--> <!-->mg<!--> <!-->TPH<sub>d</sub>/dry soil to 3026, 1851, 4105, and 6506<!--> <!-->mg<!--> <!-->TPH<sub>d</sub>/kg dry soil by Columns NE, BA, BS, and SS, respectively. The diesel-degradation efficiency was 67%, 80%, 45%, and 24%, accordingly. Microbial diversity was monitored with molecular biotechnology DGGE. It was found that introduced bacteria CC-CF3 and CC-JG39 become undetectable after 90 days of operation, but another introduced bacteria, CC-RS1, evidently remained with small signals during the last time stage of operation.</p></div>","PeriodicalId":17285,"journal":{"name":"Journal of The Chinese Institute of Chemical Engineers","volume":"39 5","pages":"Pages 419-428"},"PeriodicalIF":0.0000,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jcice.2008.03.006","citationCount":"40","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of The Chinese Institute of Chemical Engineers","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0368165308000592","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 40
Abstract
This study simulated in situ bioremediation for diesel-contaminated soil by a column operation. Several bioremediation approaches were conducted to examine the feasibility. Four lab-scale soil columns were operated specific to the following approaches: nutrient enhancement (NE), bioaugmentation (BA), biostimulation (BS), and sterilized soil (SS). Within 330 days of operation, the residual total petroleum hydrocarbon-diesel (TPHd) was degraded from 10,290 mg TPHd/dry soil to 3026, 1851, 4105, and 6506 mg TPHd/kg dry soil by Columns NE, BA, BS, and SS, respectively. The diesel-degradation efficiency was 67%, 80%, 45%, and 24%, accordingly. Microbial diversity was monitored with molecular biotechnology DGGE. It was found that introduced bacteria CC-CF3 and CC-JG39 become undetectable after 90 days of operation, but another introduced bacteria, CC-RS1, evidently remained with small signals during the last time stage of operation.