Determining the Bioactive Constituents in Calotropis gigantea Leaves by GC-MS, HPLC and FTIR Techniques

New Visions in Biological Science Vol. 1 Pub Date : 2021-08-10 DOI:10.9734/bpi/nvbs/v1/1788c

S. Uthirasamy, T. Chitra, A. Murugan, G. Manjula, P. Arulmanickam, T. Kavitha, M. Thinakaran

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引用次数: 3

Abstract

Medicinal plants have initiated in many countries because of their contributions to health care. Calotropis gigantea is a biological ingredient with potential therapeutic qualities that belongs to the Asclepiadaceae family.Calotropis gigantea leaves have long been used to treat abdominal pain, tumours, skin problems, wounds, and insect stings.This plant's therapeutic characteristics make it an important source of a medicinal compound. Calotropis gigantean's bioactive components were studied using GC-MS, HPLC, and FTIR. Gas Chromatography-Mass Spectroscopy (GC-MS) study reveals major bioactive chemicals. Only leaves contained the chemical compounds Androstane-11,17-Dione, 3-[(Trimethylsilyl)Oxy]-, 17-[O-(Phenylmethyl)Oxime], 3.Alpha.-(Trimethylsiloxy)Cholest-5-Ene, Urs-12-EN-28-Oic acid, 3-Hydroxy-, Methyl ester, (3.BETA.)-, Pseduosarsasapogenin-5, 20-Dien Methyl Ether, Beta. Carotene, 1.Alpha.,2.Alpha.-Epoxy-1.Beta.-Methylcholesta-4,6-Dien-3-One, 3-O-Acetyl-6-Methoxy-Cycloartenol whereas the rest of the compounds were similar in plants.

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GC-MS、HPLC和FTIR技术测定巨角山茶叶中生物活性成分

由于药用植物对医疗保健的贡献，许多国家已经开始使用药用植物。巨角鹿角是一种具有潜在治疗价值的生物成分，属于牛角鹿角科。巨角茶叶长期以来被用来治疗腹痛、肿瘤、皮肤问题、伤口和昆虫叮咬。该植物的治疗特性使其成为药用化合物的重要来源。采用气相色谱-质谱、高效液相色谱、红外光谱等方法对巨角牛角的生物活性成分进行了研究。气相色谱-质谱(GC-MS)研究揭示了主要的生物活性化学物质。只有叶片含有以下化合物:雄甾烷-11,17-二酮，3-[(三甲基硅氧基)氧]-，17-[O-(苯基甲基)肟]，3- α -(三甲基硅氧基)胆碱-5-烯，rs-12- en -28- oic酸，3-羟基-，甲酯，(3. β)-， pseudoosarsasapogenin - 5,20 -二烯甲基醚，β。1.α-胡萝卜素,2. alpha. -环氧- 1. - beta。-甲基胆碱-4,6-二烯-3- 1,3 - o -乙酰-6-甲氧基环青蒿醇，而其余化合物在植物中相似。

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New Visions in Biological Science Vol. 1

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