{"title":"Issue Information TOC","authors":"","doi":"10.1002/cpmb.94","DOIUrl":null,"url":null,"abstract":"<p><b>Cover</b>: Related to Laughery and Wyrick (https://doi.org/10.1002/cpmb.110), The cover image shows the experimental strategy for simple CRISPR genome editing in yeast. The central image depicts how Cas9-induced DNA breaks can be used to generate marker-free genome edits in yeast by homologous recombination (HR) with a repair template. The outer circle depicts the organization of a Cas9/sgRNA expression vector used for simple CRISPR/Cas9 genome editing in yeast.\n\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure></p>","PeriodicalId":10734,"journal":{"name":"Current Protocols in Molecular Biology","volume":"129 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmb.94","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Molecular Biology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpmb.94","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Cover: Related to Laughery and Wyrick (https://doi.org/10.1002/cpmb.110), The cover image shows the experimental strategy for simple CRISPR genome editing in yeast. The central image depicts how Cas9-induced DNA breaks can be used to generate marker-free genome edits in yeast by homologous recombination (HR) with a repair template. The outer circle depicts the organization of a Cas9/sgRNA expression vector used for simple CRISPR/Cas9 genome editing in yeast.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
