Cloning and sequence analysis of fumarase and superoxide dismutase genes from an extreme thermophile Thermus thermophilus HB27

Takehide Kosuge, Kaho Umehara, Takayuki Hoshino
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引用次数: 3

Abstract

Class II fumarase (fumC) and Mn-containing superoxide dismutase (sodA) genes were cloned and sequenced from Thermus thermophilus HB27. The fumC and sodA genes existed in tandem. Comparison of the DNA and amino acid sequences of the funC-sodA regions between T. thermophilus HB27 and Thermus aquaticus YT1 revealed that coding regions showed high homology but noncoding regions were less homologous between the two strains. The deduced amino acid sequences of T. thermophilus fumC and sodA genes showed 58.2% and 53.2% identities to those of the corresponding Escherichia coli genes, respectively. The T. thermophilus fumC gene was expressed in E. coli and the heat-resistant fumarase activity was detected at 70°C.

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极端嗜热菌HB27富马酸酶和超氧化物歧化酶基因的克隆与序列分析
从嗜热热菌HB27中克隆了II类富马酸酶(fumC)和含锰超氧化物歧化酶(sodA)基因并进行了测序。func和sodA基因是串联存在的。对嗜热T. HB27和水热T. YT1的DNA和氨基酸序列进行比较发现,编码区同源性较高,非编码区同源性较差。所得的嗜热T. fumC和sodA基因的氨基酸序列与大肠杆菌基因的同源性分别为58.2%和53.2%。在大肠杆菌中表达了嗜热T. fumC基因,并在70℃下检测了其耐热富马酸酶活性。
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