Morphometric Measurements, Meristic Counts, and Molecular Identification of Alestes Dentex, Alestes Baremoze, Brycinus Nurse, and Brycinus macrolepidotus from the River Nile at Kreima

A. T. M. Mohammed, Z. Mahmoud, H. M. Abushama
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引用次数: 1

Abstract

Twenty-two morphometric characters and seven meristic counts were recorded from 324 Alestes specimens from Kreima at the River Nile. Specimens collected were identified to the species level as Alestes baremoze (100 specimens), Brycinus nurse (100 specimens), Brycinus macrolepidotus (24 specimens), and Alestes dentex (100 specimens). The principal component analysis showed that the lateral line scales and the scale above the lateral line contributed to the percentage variance by 97.01% and 2.56%, respectively. Discriminant function analysis was done to discriminate between field-collected samples of Alestes sp. The LLS, AFR, and LDFL (Longest of Dorsal Fin Lings) were found to be the main characters that discriminate between the four populations. The first function showed that they were significantly different. This discrimination was a complete one since 98.1% of original grouped specimens and 97.8% of the cross-validated ones were correctly classified. The scatter plot of Discriminant scores from the three functions showed some relatedness between Alestes baremoze and Alestes dentex. 16S ribosomal RNA primers have been used to identify the species at the molecular level. All species have shown a characteristic band (680 bp) indicating successful amplification. Five RAPD primers have been used to investigate the Alestes species. Similar and dissimilar DNA bands indicate the evolutionary connections and genetic spacing, respectively. The derived dendrograms based on morphometric measurements and meristic counts were closer to that derived from the RAPD PCR results. The study showed co-existence of four Alestes species in the main River Nile.
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尼罗河克雷玛地区长齿Brycinus Dentex, Alestes Baremoze, Brycinus Nurse和macrolepidotus的形态测量、分生计数和分子鉴定
从尼罗河Kreima的324个Alestes标本中记录了22个形态特征和7个分生计数。采集到的标本按物种水平鉴定为白斑Brycinus baremoze(100份)、护理Brycinus(100份)、巨鳞Brycinus macrolepidotus(24份)和齿形bryestes dentex(100份)。主成分分析表明,侧线尺度和侧线以上尺度对百分比方差的贡献率分别为97.01%和2.56%。通过判别函数分析,发现LLS、AFR和LDFL(最长背鳍)是4个种群间的主要判别特征。第一个函数显示它们有显著的不同。该判别是完全的,98.1%的原始分组标本和97.8%的交叉验证标本被正确分类。3个函数的判别分数散点图显示,白菖蒲和长叶菖蒲之间存在一定的相关性。利用16S核糖体RNA引物在分子水平上对该物种进行鉴定。所有物种均有一个特征带(680 bp),表明扩增成功。利用5条RAPD引物对Alestes属植物进行了研究。相似和不相似的DNA条带分别表示进化联系和遗传间隔。基于形态测量和分生计数得出的树形图与RAPD PCR结果更接近。该研究表明,在尼罗河主河中有四种阿勒斯泰斯共存。
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