Effect of chronic exposure on the concentration of NF-kB in peripheral blood lymphocytes

Abstract

AIM: The objective of the study was to analyze the concentration of NF-kB transcription factor in lysates of peripheral blood lymphocytes of people exposed to chronic radiation during the implementation of carcinogenic effects of exposure. MATERIAL AND METHODS: The intracellular concentration of NF-kB was determined by enzyme immunoassay in protein concentration-normalized (500g/mL) lysates of peripheral blood lymphocytes. 50 chronically exposed people were examined, whose average age was 73.7 years. The average accumulated radiation dose to the red bone marrow was 727.979.1 mGy; the average accumulated exposure dose to the thymus and peripheral lymphoid organs was 85.913.6 mGy. The comparison group did not statistically significant differ from the main group in terms of age, gender, ethnic composition, and included 25 people who were not accidentally exposed. RESULTS: The median concentration of NF-kB in the main group was 34.5(27.042.4)pg/ml, in the comparison group 28.1 (25.039.3) pg/ml. CONCLUSIONS: No statistically significant differences were found in the indices in people from the main group relative to the comparison group, between different dose groups, as well as between dose groups and the comparison group. No dependences of NF-kB concentration on the exposure dose to the red bone marrow, thymus and peripheral lymphoid organs, age, gender, ethnicity of the examined people were detected.