Design and automation of an electrospinning system to prepare micro and nanofibers. Case study: elaboration of polymeric micro and nanofibers for vaginal drug delivery

Abstract

In the present investigation was optimized and automated a prototype of an electrospinning system. In addition, the methodology for preparing the polymeric film with polycaprolactone micro and nanofibers (PCL) loaded with Neem extract was optimized as a proposal for the treatment of cervical cancer. Also, a UV-VIS spectrophotometric method was developed for the quantification of Neem extract encapsulated in PCL polymeric nanofibers through the formation of a colorimetric complex with FeCl3. The wavelength used to quantify the Neem extract was 423 nm. The prototype built allowed the formation of nanofibers loaded with Neem extract with a diameter of 22-71 nm in diameter. The encapsulation efficiency of the Neem extract was 78.4%.