Comparison of antimicrobial effect of selenium nanoparticles and silver nanoparticles coated orthodontic mini-implants – An in vitro study

Abstract

Mini-implants have earned a significant role in orthodontic treatment, by augmenting anchorage requirements. Peri-implantitis contributes to miniscrew failures where progressive peri-implant bone loss occurs in conjunction with soft-tissue inflammation due to the growth of microorganisms such as Streptococcus and Lactobacillus. Nanoparticles have increased surface area and have increased interactions with biological targets like bacteria. This study aims to investigate the antimicrobial activity of silver nanoparticles (AgNP) and selenium nanoparticles (SeNPs) on orthodontic mini-implants. Mini-implant (Ti-6Al-4V) was coated with AgNP and SeNP with biopolymer (Ti-BPAgNP and Ti-BPSeNP) by dip-coating technique. The crystal structure and crystallite size of AgNPs and SeNPs were characterized by the X-ray diffraction (XRD) method. The size distribution and morphology of SeNP and AgNP were determined by a scanning electron microscope (SEM). The antibacterial activity of Ti-BP-AgNP and Ti-BPSeNP was detected from the zone of inhibition by disk diffusion assay. The SEM image of AgNP was roughly spherical, uniformly distributed and SeNPs were spherical, well distributed on the biopolymer surface. The area of the zone of inhibition of Ti-BP-SeNP-coated mini-implants shows a negligible difference in antibacterial activity compared to Ti-BPAgNP-coated mini-implants. Ti-BP-AgNP and Ti-BP-SeNP showed that a strong antibacterial activity was against Lactobacillus and Staphylococcus aureus. Antibacterial activity against Streptococcus mutans was slightly less than observed in other bacteria. SeNP shows only a marginal difference in antibacterial activity when compared to AgNP.