Immobilization of lipase on mesoporous silica nanoparticles with hierarchical fibrous pore

Q2 Chemical Engineering Journal of Molecular Catalysis B-enzymatic Pub Date : 2016-12-01 DOI:10.1016/j.molcatb.2016.10.011

Zafar Ali, Lei Tian, Panpan Zhao, Baoliang Zhang, Nisar Ali, Muhammad Khan, Qiuyu Zhang

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引用次数: 49

Abstract

Lipase from Candida Ragusa (CRL) was successfully covalently immobilized on fibrous silica nanoparticles KCC-1, and the properties of immobilized enzyme were investigated. Mesoporous fibrous silica nanoparticles (MSNPs) were synthesized with particles size 200 nm pore size 15–30 nm; followed by amino-functionalization. Scanning Electron Microscopy (SEM), Transmittance Electronic microscopy (TEM), Fourier Transform Infrared Spectroscopy (FT-IR) and N₂ adsorption were used for the characterization of nanoparticles. Further SiO₂@NH₂ nanoparticles were activated by glutaraldehyde as a bifunctional cross linker, and were used for lipase immobilization. The applied approach for support preparation, activation, and optimization of immobilization conditions, led to better resistance to temperature and pH inactivation in comparison to the free lipase, and hence widened the reaction pH and temperature regions, with the optimum pH and temperature of 7.5 and 40 °C, respectively. The immobilized Lipase Candida Ragusa (ICRL) maintained above 81% of the initial activity after 28 days and 80% activity after 8 repeated cycles. Thus ICRL showed improved storage stability reusability and 700 U/g of protein as immobilization efficiency.

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具有分层纤维孔的介孔二氧化硅纳米颗粒固定化脂肪酶

将假丝酵母(Candida Ragusa, CRL)脂肪酶共价固定在二氧化硅纤维纳米颗粒KCC-1上，研究了固定化酶的性质。合成了粒径为200 nm、孔径为15 ~ 30 nm的介孔二氧化硅纤维纳米颗粒(MSNPs);然后是氨基功能化。利用扫描电镜(SEM)、透射电镜(TEM)、傅里叶变换红外光谱(FT-IR)和N2吸附对纳米颗粒进行表征。进一步的SiO2@NH2纳米颗粒被戊二醛作为双功能交联剂激活，并用于固定化脂肪酶。通过载体制备、活化和固定化条件的优化，使其比游离脂肪酶具有更好的温度和pH失活能力，从而扩大了反应的pH和温度范围，最适pH和温度分别为7.5℃和40℃。固定化假丝酵母脂肪酶(ICRL) 28 d后活性维持在初始的81%以上，8个循环后活性维持在80%以上。结果表明，ICRL具有较高的储存稳定性和可重复使用性，固定效率为700 U/g。

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来源期刊

Journal of Molecular Catalysis B-enzymatic 生物-生化与分子生物学

CiteScore

2.58

自引率

0.00%

发文量

审稿时长

3.4 months

期刊介绍： Journal of Molecular Catalysis B: Enzymatic is an international forum for researchers and product developers in the applications of whole-cell and cell-free enzymes as catalysts in organic synthesis. Emphasis is on mechanistic and synthetic aspects of the biocatalytic transformation. Papers should report novel and significant advances in one or more of the following topics; Applied and fundamental studies of enzymes used for biocatalysis; Industrial applications of enzymatic processes, e.g. in fine chemical synthesis; Chemo-, regio- and enantioselective transformations; Screening for biocatalysts; Integration of biocatalytic and chemical steps in organic syntheses; Novel biocatalysts, e.g. enzymes from extremophiles and catalytic antibodies; Enzyme immobilization and stabilization, particularly in non-conventional media; Bioprocess engineering aspects, e.g. membrane bioreactors; Improvement of catalytic performance of enzymes, e.g. by protein engineering or chemical modification; Structural studies, including computer simulation, relating to substrate specificity and reaction selectivity; Biomimetic studies related to enzymatic transformations.